Fruit texture is a major target of apple (Malus domestica) breeding programs due to its influence on consumer preference. This multitrait feature is typically rated using sensory assessment, which is subjective and prone to biases. Instrumental measurements have predominantly targeted firmness of the outer region of fruit cortex using industry standard Magness–Taylor-type penetrometers, while other metrics remain largely unused. Additionally, there have been limited reports on correlating sensory attributes with instrumental metrics on many diverse apple selections. This report is the first to correlate multiyear historical fruit texture information of instrumental metrics and sensory assessment in an apple breeding program. Through 11 years of routine fruit quality evaluation at the Washington State University apple breeding program, physical textural data of 84,552 fruit acquired from computerized penetrometers were correlated with sensory assessment. Correlations among various instrumental metrics are high (0.63 ≤ r ≤ 1.00; P < 0.0001). In correlating instrumental outputs with sensory data, there is a significant correlation (r = 0.43; P < 0.0001) between the instrumental crispness value and sensory crispness. Additionally, instrumental hardness traits are significantly correlated (0.61 ≤ r ≤ 0.69; P < 0.0001) with sensory hardness. Outputs from two versions of computerized penetrometers were tested and shown to have no statistical differences. Overall, this report demonstrates potential use of instrumental metrics as firmness and crispness estimates for selecting apples of diverse backgrounds in a breeding program. However, in testing a large number and diversity of fruit, experimenters should perform data curation and account for lower limits/thresholds of the instrument.
Washington State University's apple breeding program (WABP) was initiated in 1994 to select new apple cultivars with improved eating quality, appearance, and storability that are suitable for production in the main growing regions of the state. Fruit quality is phenotyped using various instrumental measures, such as penetrometers (texture), titrator (acidity), and refractometer (soluble solids concentration; SSC), as well as sensory assessment. The selection regime of WABP occurs in three sequential phases: phase one (P1)—single, unreplicated seedlings at one site, phase two (P2)—replicated selections at three geographically diverse sites, and phase three (P3)—highly replicated elite selections at one to two grower sites. Most of the data collection of WABP occurs in P2. Knowledge of trends/changes associated with advancing selections is essential for understanding the selection criteria and progress of WABP throughout the changing compositions of advancing and culling selections. For each post-harvest trait, P2 data from harvest years 2005 to 2019 were split across sites, and between selections and reference cultivars (e.g., Cripps Pink, Gala, and Honeycrisp). Means of instrumental crispness (Cn) and inner cortex firmness for the advancing selections increased gradually over this period and were significantly higher than those for cultivars. Means of outer cortex firmness measurements were stable for selections but significantly higher than those for cultivars. The average fruit acidity of selections increased marginally over this period and was higher than that of the cultivars. Meanwhile, the average fruit SSCs of selections and cultivars were statistically indistinguishable. These 15-year trends indicate that WABP has been selecting apples with improved eating quality and storability through increased crispness and inner cortex firmness, respectively.
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