Background: In this study, we investigated the effect of hippocampal subfield atrophy on the development of Alzheimer’s disease (AD) by analyzing baseline magnetic resonance images (MRI) and images collected over a one-year follow-up period. Previous studies have suggested that morphological changes to the hippocampus are involved in both normal ageing and the development of AD. The volume of the hippocampus is an authentic imaging biomarker for AD. However, the diverse relationship of anatomical and complex functional connectivity between different subfields implies that neurodegenerative disease could lead to differences between the atrophy rates of subfields. Therefore, morphometric measurements at subfield-level could provide stronger biomarkers. Methods: Hippocampal subfield atrophies are measured using MRI scans, taken at multiple time points, and shape-based normalization to a Montreal neurological institute (MNI) ICBM 152 nonlinear atlas. Ninety subjects were selected from the Alzheimer’s Disease Neuroimaging Initiative (ADNI), and divided equally into Healthy Controls (HC), AD, and mild cognitive impairment (MCI) groups. These subjects underwent serial MRI studies at three time-points: baseline, 6 months and 12 months. Results: We analyzed the subfield-level hippocampal morphometric effects of normal ageing and AD based on radial distance mapping and volume measurements. We identified a general trend and observed the largest hippocampal subfield atrophies in the AD group. Atrophy of the bilateral CA1, CA2- CA4 and subiculum subfields was higher in the case of AD than in MCI and HC. We observed the highest rate of reduction in the total volume of the hippocampus, especially in the CA1 and subiculum regions, in the case of MCI. Conclusion: Our findings show that hippocampal subfield atrophy varies among the three study groups.

Background: In this study, we investigated the fusion of texture and morphometric features as a possible diagnostic biomarker for Alzheimer’s Disease (AD). Methods: In particular, we classified subjects with Alzheimer’s disease, Mild Cognitive Impairment (MCI) and Normal Control (NC) based on texture and morphometric features. Currently, neuropsychiatric categorization provides the ground truth for AD and MCI diagnosis. This can then be supported by biological data such as the results of imaging studies. Cerebral atrophy has been shown to correlate strongly with cognitive symptoms. Hence, Magnetic Resonance (MR) images of the brain are important resources for AD diagnosis. In the proposed method, we used three different types of features identified from structural MR images: Gabor, hippocampus morphometric, and Two Dimensional (2D) and Three Dimensional (3D) Gray Level Co-occurrence Matrix (GLCM). The experimental results, obtained using a 5-fold cross-validated Support Vector Machine (SVM) with 2DGLCM and 3DGLCM multi-feature fusion approaches, indicate that we achieved 81.05% ±1.34, 86.61% ±1.25 correct classification rate with 95% Confidence Interval (CI) falls between (80.75-81.35) and (86.33-86.89) respectively, 83.33%±2.15, 84.21%±1.42 sensitivity and 80.95%±1.52, 85.00%±1.24 specificity in our classification of AD against NC subjects, thus outperforming recent works found in the literature. For the classification of MCI against AD, the SVM achieved a 76.31% ± 2.18, 78.95% ±2.26 correct classification rate, 75.00% ±1.34, 76.19%±1.84 sensitivity and 77.78% ±1.14, 82.35% ±1.34 specificity. Results and Conclusion: The results of the third experiment, with MCI against NC, also showed that the multiclass SVM provided highly accurate classification results. These findings suggest that this approach is efficient and may be a promising strategy for obtaining better AD, MCI and NC classification performance.

Background: In this study, we used a convolutional neural network (CNN) to classify Alzheimer’s disease (AD), mild cognitive impairment (MCI), and normal control (NC) subjects based on images of the hippocampus region extracted from magnetic resonance (MR) images of the brain. Materials and Methods: The datasets used in this study were obtained from the Alzheimer's Disease Neuroimaging Initiative (ADNI). To segment the hippocampal region automatically, the patient brain MR images were matched to the International Consortium for Brain Mapping template (ICBM) using 3D-Slicer software. Using prior knowledge and anatomical annotation label information, the hippocampal region was automatically extracted from the brain MR images. Results: The area of the hippocampus in each image was preprocessed using local entropy minimization with a bi-cubic spline model (LEMS) by an inhomogeneity intensity correction method. To train the CNN model, we separated the dataset into three groups, namely AD/NC, AD/MCI, and MCI/NC. The prediction model achieved an accuracy of 92.3% for AD/NC, 85.6% for AD/MCI, and 78.1% for MCI/NC. Conclusion: The results of this study were compared to those of previous studies, and summarized and analyzed to facilitate more flexible analyses based on additional experiments. The classification accuracy obtained by the proposed method is highly accurate. These findings suggest that this approach is efficient and may be a promising strategy to obtain good AD, MCI and NC classification performance using small patch images of hippocampus instead of whole slide images.

Background: We propose a classification method for Alzheimer’s disease (AD) based on the texture of the hippocampus, which is the organ that is most affected by the onset of AD. Methods: We obtained magnetic resonance images (MRIs) of Alzheimer’s patients from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) dataset. This dataset consists of image data for AD, mild cognitive impairment (MCI), and normal controls (NCs), classified according to the cognitive condition. In this study, the research methods included image processing, texture analyses, and deep learning. Firstly, images were acquired for texture analyses, which were then re-spaced, registered, and cropped with Gabor filters applied to the resulting image data. In the texture analyses, we applied the 3-dimensional (3D) gray-level co-occurrence (GLCM) method to evaluate the textural features of the image, and used Fisher’s coefficient to select the appropriate features for classification. In the last stage, we implemented a deep learning multi-layer perceptron (MLP) model, which we divided into three types, namely, AD-MCI, AD-NC, and MCI-NC. Results: We used this model to assess the accuracy of the proposed method. The classification accuracy of the proposed deep learning model was confirmed in the cases of AD-MCI (72.5%), ADNC (85%), and MCI-NC (75%). We also evaluated the results obtained using a confusion matrix, support vector machine (SVM), and K-nearest neighbor (KNN) classifier and analyzed the results to objectively verify our model. We obtained the highest accuracy of 85% in the AD-NC. Conclusion: The proposed model was at least 6–19% more accurate than the SVM and KNN classifiers, respectively. Hence, this study confirms the validity and superiority of the proposed method, which can be used as a diagnostic tool for early Alzheimer’s diagnosis.