Background: Surgical mortality data are collected routinely in high-income countries, yet virtually no low-or middle-income countries have outcome surveillance in place. The aim was prospectively to collect worldwide mortality data following emergency abdominal surgery, comparing findings across countries with a low, middle or high Human Development Index (HDI).Methods: This was a prospective, multicentre, cohort study. Self-selected hospitals performing emergency surgery submitted prespecified data for consecutive patients from at least one 2-week interval during July to December 2014. Postoperative mortality was analysed by hierarchical multivariable logistic regression.
Puccinia triticina, Puccinia graminis and Puccinia striiformis cause leaf, stem and yellow rust, respectively. Wheat rusts can cause losses as high as 70%. The rusts ability to evolve fungicide resistance has resulted in the use of resistant cultivars as the primary method of control. Breeding resistant cultivars is a long process and requires an accurate picture of the current and future pathogen population. Differentiation of wheat rust pathotypes using conventional plant pathology techniques is time consuming, labour intensive and requires the services of a highly skilled and experienced plant pathologist. Modern molecular biology techniques have the potential to aid the conventional techniques and provide fast, accurate same-day results. Microsatellite markers were used to differentiate P. triticina and P. striiformis pathotypes. Amplified fragment length polymorphisms (AFLP) were used to differentiate stem rust P. graminis pathotypes. Phylogenetic trees were created for leaf and stem rust pathotypes. Field isolates of leaf, stem and yellow rust were collected from eleven sites across the Western Cape Province. Microsatellite markers were used to type leaf and yellow rust isolates. AFLP markers could not be used on field isolates due to the presence of plant DNA. Novel alleles found in the Leaf and yellow rusts isolates prevented the assigning of a specific pathotype to each isolate. UVPrt10 (25.2%) and UVPrt9 (21.5%) were the most prevalent leaf rust pathotypes. Only 6E16A+ was identified in the yellow rust isolates. Pathotype incidence was similar to previous studies. The prevalence of multiple pathotypes with a variety of virulence genes in the rust population shows that breeding lines with single major resistance genes will not be effective and breeding programmes should concentrate on lines that exhibit quantitative resistance.
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