Environmental pollution by heavy metals is a recent global problem threatening human life through the food chain. The used of fungi provides an alternative solution to eliminate these heavy metals. The current study aims to study the change of secondary metabolic pathways of fungal strain tolerant of Zinc metal. Penicillium chrysogenum selected strain was isolated from the sand of beaches polluted with Zinc at East Algerian area. The optimization of Penicillium chrysogenum strain was carried out on Czapek Yeast Agar medium (CYA) at different temperature and pH values. The effect of Zinc on the production of secondary metabolites by Penicillium chrysogenum strain was determined on solid medium, Thin Layer Chromatography (TLC) plate and High Performance Liquid Chromatography (HPLC). The results revealed that Penicillium chrysogenum strain can develop in a wide range of temperature and pH with an optimum of 28 ° C and 6. It is capable to tolerate high concentrations of Zinc (up to 1400 mg.l-1).This study showed that high concentrations of Zinc (1200 and 1400 mg.l-1) have a positive effect on antibiotic production, but they have a negative effect on the mycotoxin production except to neoxaline.