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The objective of this study was to evaluate the effects of banana flower power pellet (BAFLOP-pellet) and plant oil source on in vitro gas production, fermentation efficiency, and methane (CH) production. Rumen fluid was collected from two rumen-fistulated dairy steers fed on rice straw-based diet with concentrate supplement to maintain normal rumen ecology. All supplemented feed were added to respective treatments in the 30:70 roughage to concentrate-based substrate. The treatments were arranged according to a 3 × 3 factorial arrangement in a completely randomized design. First factor was different levels of BAFLOP-pellet supplementation (0, 30, and 60 g/kg of dietary substrate) and second factor was plant oil source supplementation [non-supplemented, 20 g/kg krabok seed oil (KSO), and 20 g/kg coconut oil (CO) of dietary substrate, respectively]. Under this investigation, BAFLOP-pellet supplementation increased gas production kinetics and in vitro digestibility (P < 0.05). Ruminal pH was dropped post incubation time in the non-supplemented group but was enhanced in BAFLOP-pellet-supplemented treatments. On the other hand, supplementation of KSO and CO depressed gas production and digestibility, but did not influence ruminal pH. In addition, protozoal population and CH production were decreased by BAFLOP-pellet and plant oil addition (P < 0.05). Based on this study, it could be concluded that supplementation of BAFLOP-pellet and plant oil source could enhance the in vitro fermentation efficiency while reduced protozoal population and CH production. It is suggested that BAFLOP-pellet (60 g/kg of dietary substrate) and KSO/CO (20 g/kg of dietary substrate) could be used to manipulate rumen fermentation characteristics fed on high-concentrate diet.

High-quality protein roughage is an important feed for productive ruminants. This study examined the effects of strategic feeding of lactating cows with cassava (Manihot esculenta) top silage (CTS) on rumen fermentation, feed intake, milk yield, and quality. Four early lactating crossbred dairy cows (75% Holstein-Friesian and 25% Thai) with body weight (BW) 410 ± 30 kg and milk yield 12 ± 2 kg/day were randomly allotted in a 4 × 4 Latin square design to four different supplementation levels of CTS namely, 0, 0.75, 1.50, and 2.25 kg/day of dry matter (DM). Strategic supplementation of CTS significantly affected ruminal fermentation end-products, especially increased propionate production, decreased protozoal population and suppressed methane production (P < 0.05). Increasing the CTS supplementation level substantially enhanced milk yield and the 3.5% FCM from 12.7 to 14.0 kg/day and from 14.6 to 17.2 kg/day (P < 0.05) for non-supplemented group and for the 2.25 kg/day supplemented group, respectively. We conclude that high-quality protein roughage significantly enhances rumen fermentation end-products, milk yield, and quality in dairy cows.

Fodder shrub, Flemingia macrophylla silage (FMS), was nutritionally evaluated for rumen fermentation characteristics using an in vitro gas production technique. Dietary treatments were imposed in a 2 × 7 factorial arrangement in a completely randomised design. The first factor was two ratios of roughage:concentrate (R:C at 60:40 and 40:60), and the second factor was seven levels of FMS supplementation at 0, 20, 40, 60, 80, 100 and 120 g/kg of dietary substrate. The results revealed that a R:C ratio at 40:60 increased the kinetics of gas production (P &lt; 0.001). Moreover, supplementation of FMS increased in vitro degradability and the concentration of propionic acid (P &lt; 0.001), whereas acetic acid concentration, protozoal population and methane production were reduced (P &lt; 0.001). In conclusion, supplementation of FMS at 120 g/kg of dietary substrate improved rumen fermentation and subsequently reduced methane production. However, in vivo feeding trials using FMS should be conducted to gain more information for further implementations.

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