A central point adding to the capacity of organizations to adapt to the turbulent environment is the advancement of job satisfaction and employee well-being. The purpose of this study was to understand the effect of gender on the job satisfaction and psychological well-being of the employees working in Indian private sector. Consequently, the relationship between job satisfaction and well-being was explored. A cross-sectional survey-based research design was adopted using data from 160 employees (equal number of males and females) of private sector organizations in North India. The Minnesota Satisfaction Questionnaire (MSQ) and The Warwick-Edinburgh Mental Well-being Scale (WEMWBS) were administered to gain insight into the levels of job satisfaction and well-being respectively. The results showed that gender did not play a role in determining job satisfaction and psychological well-being in Indian employees. Further, it was seen that job satisfaction and psychological well-being were positively correlated. The study advances the existing work and adds to the limited literature of Indian studies on the relationship between job satisfaction and well-being in the Indian private sector.