The aim of this article was to determine the prevalence of the Republic of Croatia preschool teachers’ physical activity level and its correlation to chronological age, years of service, degree of education, and place of work. The research was conducted on a sample of 159 preschool teachers from different parts of the Republic of Croatia. The Croatian version of the standardised version of the International Physical Activity Questionnaire (IPAQ) was used. The basic descriptive parameters were calculated, while the correlation between certain variables was determined by the Spearman correlation coefficient and linear regression analysis. Results have shown that almost 80% of preschool teachers do not meet the criteria for the recommended levels of physical activity. They are most physically active at work but are the least active during transportation and leisure time. A statistically significant correlation was determined between physical activity and age, years of service, and place of work. Younger preschool teachers are significantly more physically active in their leisure time than older ones, as are those with fewer years of service. Preschool teachers who work in a bigger city are more physically active at work, whereas those from smaller cities are more active doing their household chores. Furthermore, the independent variables (Age, Years of service, Place of work, Degree of education) in combination significantly affect the level of physical activity of preschool teachers in their leisure time and the overall level of physical activity. Preschool teachers should be models for children, and if they are not physically active themselves, they will not be sufficiently motivated to stimulate the same in children, i.e., the habit of leading an active and healthy life from an early age.