Purpose To examine whether two consecutive soccer matches would affect skin temperature (t sk) measured via infrared thermography (IRT) in under-20 (U-20) soccer athletes, as well as verify whether the t sk changes would be different between regions of interest. Methods A cross sectional study. Ten under-20 soccer athletes [age 19.0 ± 1.0 years; height 181.3 ± 6.6 cm; body fat percentage (BF%) 9.0 ± 1.8%, body surface area 1.9 m 2 and _ VO 2max 56.4 ± 3.2 ml min-1 kg-1 ]. Skin thermal responses obtained by IRT and creatine kinase concentration (CK) were evaluated in response to two soccer matches with 3 days of recovery between each match. Results The t sk increased (%1.0°C) 24 h after the first match in all studied regions of interest (ROIs), returning to near pre-match values 48 h after the first match. However, after playing the second match, the t sk increased even more (%1.5°C) 24 h after in all the considered ROIs, not returning to pre-match values 48 h after. Regarding CK analysis, its course during the experiment was similar to t sk , with high values 24 h after (first match 763.8 ± 294.5 U/ L; second match 784.1 ± 298.8 U/L) and recovering 48 h after (first match 526.4 ± 289.7 U/L; second match 672.2 ± 285.0 U/L) both matches. However, when the two matches are compared, CK showed a higher value 48 h after the second match in comparison to first match (P = 0.002). The t sk and CK were moderately correlated in all ROIs analyzed, with higher correlation in the anterior right leg (r = 0.425) and anterior left leg (r = 0.428). Conclusion The t sk of lower limbs as well as CK markedly change in response to two consecutive matches separated by an interval of 3 days. There is indication of the highest inflammatory response after the second match, which was preceded by just a 3-day recovery. In addition, a general increase was obtained in thighs and legs in anterior and posterior views.