Background: A microbiological analysis of samples taken from hands of food handlers and utensils/crockery in food service units was used to assess the risk of food contamination. We aimed to assess indicators of microbiological contamination in different food service units. Methods: We used data for an official inspection proposal (2018–2019) in different food service units serving a district in Northeast Portugal. We showed the proportion of positive samples for each microorganism assessed in samples taken from hands and kitchen utensils/crockery. Results: Out of 471 samples taken from hands of food handlers, 26.8% (n = 126) were classified as “unsatisfactory” due to the presence of at least Escherichia coli, Staphylococcus aureus and/or total and fecal coliforms. Such a proportion varied according to the type of food service unit with a higher proportion being observed in Canteens (40.0%) and in Retirement Home/Day Care Centers (37.8%). More than one third of 649 samples taken from kitchen utensils/crockery were classified as “unsatisfactory” (n = 181; 27.9%) or “bad” (n = 49; 7.6%). There were significant differences in these proportions across food service units, with a higher proportion of “unsatisfactory” samples being observed in Fishmongers and Butcher Shops/Charcuteries (50.0% and 45.1%, respectively), while a higher proportion of “bad” samples was observed in both Pastry/Bakery and Fishmonger establishments (33.3%), followed by Butcher Shops/Charcuteries (14.1%). Conclusions: Our results emphasize the need of awareness of hygiene practices among food handlers of different food service units, particularly in Fishmongers, Pastry/Bakery establishments and Butcher Shops/Charcuteries, highlighting the need of a periodic hygienic evaluation.

The screening of groups with a high risk for developing tuberculosis (TB) is a priority in order to control this disease. Since there is no gold standard for the diagnosis of latent TB infection (LTBI), both the tuberculin skin test (TST) and the interferon-gamma release assays (IGRA) have been used for this purpose. The aim of this study was to determine the proportion of LTBI by using the TST and the IGRA tests, and to assess the risk factors related with discordant results between tests across several risk groups advised for screening in Northeast Portugal. Data were collected from the database of patients with suspected LTBI and advised for the screening in a public health unit (January 2014 to December 2015). The proportion of LTBI was computed using both tests. Logistic regression models assessed risk factors for a positive test and for discordant results between tests. The adjusted odds ratio (OR) and respective 95% confidence interval (95% CI) were obtained. Out of 367 patients included in the analysis, 79.8% had a positive TST and 46.0% of them had a positive IGRA. In comparison with contacts of active TB cases, healthcare workers and inmates presented higher odds of TST positivity (OR 4.38, 95% CI 1.59–12.09 and OR 4.74, 95% CI 1.45–15.49, respectively), but immunocompromised people presented lower odds of TST positivity (OR 0.14; 95% CI 0.06–0.31). Instead, healthcare workers (OR 0.44, 95% CI 0.24–0.80) and immunocompromised people (OR 0.24, 95% CI 0.10–0.56) presented lower odds of a positive IGRA. There were 42.0% concordant positive results, 16.1% concordant negative results, and 41.9% discordant results, with healthcare workers presenting higher odds of discordant results (OR 3.34, 95% CI 1.84–6.05). The proportion of LTBI estimated by TST and IGRA among people advised for screening in our setting is high, highlighting the need of preventive strategies. Among healthcare workers, TST results should be read with caution as the higher proportion of discordant results with a positive TST suggests the impact of the booster reaction in this group.

Background The poor water quality of recreational and rehabilitation pools, has been correlated to insufficient disinfection or human contamination, and can be a vehicle for transmitting diseases to users. Because people with different characteristics attend public swimming pools with different purposes, the pool water quality control is essential to minimize hazards related to unsafe water. Methods This study was based on data collection of 144 samples between 2018 and 2020 in therapeutic and recreational pools (indoor and outdoor) in the Bragança district. The proportion of the number of bathers were estimated, as well as the microbial parameters: Total coliforms, Escherichia coli, enterococci, Pseudomonas aeruginosa, total Staphylococcus, Staphylococcus coagulase-positive and mesophilic microbial count. Results The E. coli, enterococci and mesophilic microbial count were not found in any sample. Unsafe pool water was mainly contaminated by both photogenic P. aeruginosa (6.3%) and total Sthaphylococcus (10.4%). Levels of P. aeruginosa in therapeutic pools was greater than in recreational pools (indoor and outdoor) (10.5% vs 6.8% and 4.5%, respectively), while total Sthaphylococcus was absent in therapeutic pools and represent 13.6% and 10.6% of positivity in indoor and outdoor swimming pools, respectively. The bathers number medium of therapeutic, indoor and outdoor pools was 4.8, 3.4 and 6.9, respectively. Conclusions Overall, the results endorse the good water quality of these swimming pools, mainly by the absent of faecally-derived bacteria. The presence of P. aeruginosa in therapeutic pools can be explained by the type of users, while the high number of bathers may promote the growth of some microorganisms, particularly in outdoor pools.