This study proposed using a portable microscope combined with a smartphone and application (PhotoMetrix® application) to evaluate the authenticity of Brazilian banknotes (R$50 and R$100) and national driver’s licenses (NDL) through image acquisition (Samsung S7) and chemometric treatment (principal component analysis (PCA)). Six regions of the banknote were analyzed: holographic band; lower and upper tactile regions containing the number referring to the value of the note; microprints above the effigy (obverse); and numbers and the surroundings of the animal formed by microprints (reverse). For NDLs, the regions were the following: the coat of arms of the republic; the state map with microprints; optical ink variation; distorted positive microletters with technical failure; negative guilloche; typographic numbering; micro letter wire; and region with line printing. For the chemometric study with Photometrix®, we selected a region of interest (ROI) of 32 × 32 and 64 × 64 pixels with autoscaled data using the channels red (R), green (G), blue (B), hue (H), saturation (S), value (V), lightness (L) and intensity (I). We obtained excellent results for differentiating banknotes and NDLs, both by visual and chemometric analyses (PCA). This study demonstrates the effectiveness of using a portable microscope and a smartphone as a portable forensic tool that is fast, robust, low-cost and reliable.