Background: The Mre11-Rad50 (MR) complex coordinates DNA double strand break repair. Results: Mutations of the Rad50 coiled-coil disrupt the enzymatic functions of the MR complex. Conclusion: The Rad50 coiled-coil regulates and mediates communication between the enzymatic domains of Rad50 and Mre11. Significance: The Rad50 coiled-coil plays a more direct role in the enzymatic function of Rad50 and Mre11 than was previously assumed.
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