The objective of this study was to determine if the presence of two acetolactate synthase (ALS)-inhibiting herbicide residues in different Saskatchewan soils would result in additive, synergistic, or antagonistic interactions. This was determined through field trials where herbicides were applied sequentially over the course of 2 yr. The herbicides examined in these experiments were imazamethabenz, flucarbazone, sulfosulfuron, and florasulam, each in combination with imazamox and imazethapyr. The phytotoxicity and persistence of the herbicides in soil was assessed using an oriental mustard root inhibition bioassay. The determination of herbicide interaction was made through the comparison of the experimentally observed values to theoretically expected values derived from a mathematical equation. On the basis of the bioassay analysis, it was found that the herbicide residue combinations resulting from sequentially applied ALS-inhibiting herbicides in the three soils produced additive injury effects rather than synergistic or antagonistic interactions.

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