Nowadays 89Zr is outstanding PET radionuclide with its physical half-life (t1/2 ~ 78 hours), useful decay specifications and so that it is suitable for antibody-based immuno-PET. Relatively oscillated positrons' low decay energies supply to take high-resolution. 89Zr-labeled radiopharmaceuticals, especially as 89Zrlabeled antibodies applications are getting increase day by day.In this study, calculations about production of 89Zr were done and used (p,n) reaction of 89Y target system. For this Q-value, Threshold Energy, Minimum Coulomb Barrier Energy of the reaction were calculated then the cross-sections of this reaction were found using Empire3.2/MALTA code. After determining the irradiation calculations, the bombardment performed. The irradiation was performed in Ankara Sarayköy Nuclear Research and Training Center, proton accelerator. The cyclotron is IBA type Cyclone-30. Then separation part was done with Dowex resine system. After separation 89Zr from the irradiated target system, radioactive 89Zr was obtained purely..