INTRODUCTION: The role of free radicals is well established as a crucial component of the immune system. AIM: To assess the perception of dental practitioners of Northern and Central India regarding the role of antioxidants in oral health.MATERIALS AND METHOD: A pre-tested and pre-validated online questionnaire was distributed among various dental practitioners in Northern and Central India which consisted of dichotomous responses. Descriptive statistics were used to summarize the data, followed by the Chi square test to check significant differences between the responses. Correlation between responses were analysed through the Spearman’s rank correlation. Statistical analysis was done using Statistical Package for Social Sciences version 20 (SPSS 20).RESULTS: A statistically significant difference was observed among the genders, with females 126 (43.7%) having lesser knowledge than males 162(56.3%) regarding the use of antioxidants in their clinical practice. High knowledge scores were observed in 288 (69.2%) dental professionals. Postgraduates were found to have (p=0.05) a higher level of knowledge as compared to graduates.CONCLUSION: The use of antioxidants in dentistry should be increased through the conduction of various CDE programmes. workshops and evidence-based practice guidelines.