A Gram-negative-staining, non-motile rod, designated GG-w14 T , was isolated from the rhizosphere of Angelica polymorpha Maxim. Phylogenetic analysis of 16S rRNA gene sequences revealed that the isolate belonged to the genus Mucilaginibacter and exhibited 93.9-97.4 % 16S rRNA gene sequence similarity with recognized members of the genus Mucilaginibacter (closest relative Mucilaginibacter gossypii Gh-67 T ). DNA-DNA relatedness between strain GG-w14 T andM. gossypii KCTC 22380 T was ,41 %. Strain GG-w14 T grew at 4-35 6C, at pH 5.0-8.0 and with 0-1 % (w/v) NaCl. The isolate hydrolysed casein, CM-cellulose and starch and contained menaquinone 7 as the major menaquinone. The major cellular fatty acids were summed feature 3 (C 16 : 1 v7c and/or iso-C 15 : 0 2-OH; 39.9 %), iso-C 15 : 0 (24.2 %) and iso-C 17 : 0 3-OH (12.4 %). The DNA G+C content was 42.5 mol%. These data suggest that strain GG-w14 T should be considered as a representative of a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter angelicae sp. nov. is proposed. The type strain is GG-w14The genus Mucilaginibacter, a member of the family Sphingobacteriaceae, was originally proposed by Pankratov et al. A novel bacterial strain, designated GG-w14 T , was isolated from the rhizosphere of Angelica polymorpha Maxim, which is used as a medicinal herb in Korean traditional medicine (Heo, 1610). Samples of A. polymorpha Maxim. were collected at Chungcheongnam-Do, Republic of Korea. Rhizosphere soil collected from the plant roots was serially diluted with sterile 0.85 % (w/v) NaCl and dilutions were spread onto R2A agar (BD). Colonies were subcultivated to obtain pure cultures. Strain GG-w14 T was one of the isolates obtained. Cell morphology and size were observed using light microscopy (Eclipse 80i; Nikon) and transmission electron microscopy (model H-7600; Hitachi) from cells cultured for 2 days at 25 uC on R2A agar. Colony morphology was observed after culture on R2A agar for 3 days at 25 u C. Growth with 0, 1, 2 and 3 % (w/v) NaCl was determined in NB (BD). Growth at 4, 10, 15, 25, 30, 35, 40 and 45 u C was determined on NA (BD). Growth at pH 4.0-9.0 (in increments of 0.5 pH unit) was tested in pH-adjusted NB. Growth was measured spectrophotometrically at OD 600 using a DU 730 UV/Vis scanning spectrophotometer (Beckman Coulter). Unless otherwise stated, all physiological characteristics were tested with cells cultured on R2A agar under optimal growth conditions (25-30 u C and pH 6.0-7.0). A Gram-stain kit (BD) was used for Gramstaining and oxidase reagent (bioMérieux) was used for the measurement of oxidase activity. Catalase activity was determined by observing the production of oxygen bubbles with 3 % (v/v) hydrogen peroxide. Anaerobic growth was

Herbiconiux moechotypicola sp. nov., a xylanolytic bacterium isolated from the gut of hairy long-horned toad beetles, Moechotypa diphysis (Pascoe) A novel Gram-positive, non-motile, rod-shaped bacterium, designated strain RB-62 T , was isolated during a study of culturable bacteria from the gut of Moechotypa diphysis (Pascoe) and its taxonomic position was investigated. Strain RB-62 T grew at 15-30 6C and pH 5.0-8.5. The isoprenoid quinones were menaquinones MK-11 (77.1 %), MK-10 (11.7 %) and MK-12 (11.2 %). The major cellular fatty acids were anteiso-C 15 : 0 (34.6 %), anteiso-C 17 : 0 (29.8 %), iso-C 16 : 0 (17.0 %) and cyclohexyl-C 17 : 0 (11.4 %). The diagnostic diamino acid of the cell-wall peptidoglycan was 2,4-diaminobutyric acid. The G+C content of the genomic DNA of strain RB-62 T was 70.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RB-62 T was affiliated with the genus Herbiconiux cluster within the family Microbacteriaceae, and was related most closely to Herbiconiux ginsengi wged11 T (98.08 % similarity). The level of DNA-DNA relatedness between strain RB-62 T and H. ginsengi wged11 T was 43.2 % (reciprocal 66.7 %). Phenotypic and phylogenetic characteristics clearly distinguished strain RB-62 T from recognized species of the genus Herbiconiux. Based on data from the present polyphasic study, strain RB-62 T is considered to represent a novel species of the genus Herbiconiux, for which the name Herbiconiux moechotypicola sp. nov. is proposed. The type strain is RB-62 T (5KCTC 19653 T 5JCM 16117 T ).