A highly sensitive immunosensor based on scanning tunneling microscopy (STM) was developed for the first time to detect living material such as HIV-1 virus by gold (Au) nanoparticle and fragmented antibody complex. Fragmented antibodies were pre-immobilized on the Au surface, then HIV-1 virus like particles (HIV-1 VLPs) and Au-nanoparticle and fragmented antibody complexes were applied to develop sandwich assay. The developed surface morphology and the current profile of fabricated immunosensing element were characterized by Raman spectroscopy and investigated with STM. The power spectrum derived from the current profile was found to be related with concentrations of HIV-1 VLPs. Using the electrical detection method based on current mapping profile of STM, living material such as virus, HIV-1 VLPs, was able to be detected successfully. The proposed technique can be a promising method to construct the highly sensitive and efficient sensor for detecting viruses and other living materials.

In order to ensure the stability of tunnel portal slope, reinforcement method such as anchors, soil nails and rock bolts have been used in Korea. When selecting slope reinforcement methods in tunnel portal area such as reinforcement arrangement and length, trial and error method can be very time-consuming and it was also not easy to verify the selection of an optimum condition. In this study, using the FISH language embedded in the finite difference code FLAC3D program, the optimization technique was developed with the Differential Evolution Algorithm (DEA). After building a database on the soil nailing method in tunnel portal area, this system can be selected to an optimum arrangement plan based on the factor of safety through the FLAC3D analysis. Through the results of numerical analysis, it was confirmed that the number of analysis was decreased by about 8 times when DEA based optimization technique was used compared to the full combination (FC). In case of the design of slope reinforcement in tunnel portal area, if this built-system is used, it is expected that the selection of an optimum arrangement plan can be relatively easier.

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