The protocol duration of tests to VO2max should be between 8 and 10 min for healthy, moderately to highly trained subjects.

Indirect calorimetry is the measurement of the amount of heat generated in an oxidation reaction by determining the intake or consumption of oxygen or by measuring the amount of carbon dioxide or nitrogen released and translating these quantities into a heat equivalent. In the last 20 years there has been significant development in both laboratory and computerized metabolic systems used in indirect calorimetry. In addition, there has been increased use of breath-by-breath EGAIC. Several researchers have suggested that breath-by-breath analysis, because of their practicality, could fulfill this need for a valid and reliable expired gas analysis indirect calorimetry instrument. It was hoped this investigation would determine the best validation for a precise measurement of breath-by-breath expired gas analysis indirect calorimetry. The problem with the available research is that few studies have examined the validity and reliability of all these different systems for breath-by-breath expired gas analysis indirect calorimetry. Therefore, there is a need to find out the most valid, reliable, and precise measurement of the breath-by-breath expired gas analysis indirect calorimetry.

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