RESUMO:O óleo essencial das folhas de Schinus terebinthifolius foi obtido por hidrodestilação e analisado por CG-EM. O rendimento do óleo foi de 0,8%, sendo identificados 37 constituintes químicos. Os componentes principais foram germacreno D (25,0%), (E)-b-cariofileno (17,5%) e d-elemeno (10,5%). O óleo essencial foi diluído em acetona nas concentrações de 10 -2 a 10 -8 e aplicado aos insetos (Hypothenemus hampei) por aplicação tópica e exposição em superfície contaminada. As taxas de mortalidade foram avaliadas após 24 e 48 horas do início do experimento. A aplicação em superfície contaminada resultou em 25% de mortalidade no controle, enquanto nas diluições de 10 -2 a 10 -8 foram observados 100,0 a 30,0% de mortalidade. Na aplicação tópica, observou-se 27,5% de mortalidade no controle e 97,5 a 77,5% nas diluições de 10 -2 a 10 -8 .Palavras-chave: cafeicultura, aroeira vermelha, inseticida natural, metabólitos secundários. ABSTRACT: Chemical composition and insecticidal activity of the essential oil ofSchinus terebinthifolius Raddi (Anacardiaceae) on coffee berry borer (Hypothenemus hampei) Ferrari. The essential oil from the leaves of Schinus terebinthifolius was obtained by hydrodistillation and was analyzed by GC-MS. The oil yield was 0.8%, and 37 chemical constituents were identified. The main components were germacrene D (25.0%), (E)-bcariophyllene (17.5%) and d-elemene (10.5%). The essential oil was diluted in acetone in concentrations of 10 -2 to 10 -8 and it was applied to insects (Hypothenemus hampei) by topical application and exposure to contaminated surface. Mortality rates were evaluated after 24 and 48 hours. The contaminated surface application resulted in 25.0% of mortality in control while the dilutions of 10 -2 to 10 -8 resulted in 100% to 30.0% of mortality respectively. In the topical application, it was observed 27.5% of mortality in control and 97.5 to 77.5% of mortality in dilutions of 10 -2 to 10 -8 .

-(Inseticidal activity of Piper hispidum (Piperaceae) leaves extract on (Hypothenemus hampei)). The objective of this work was to evaluate the insecticidal and repellent potential of the acetonic leaf extract of Piper hispidum Kunth on Hypothenemus hampei (Ferrari, 1867) insects by topical application, contaminated surface and repellent effect. Dilutions of 25.0; 5.0; 0.1; 0.02; 0.004 and 0.0008 mg mL -1 were used for exposition in contaminated surface and topical application. In the repellence test 0.5 mg mL -1 of this extract were used. Evaluations occurred during 48 hours after exposure to the extract. In the exposition in contaminated surface 100% of mortality were observed in the dilution of 25.0 mg mL -1 and 50 to 80% in dilutions of 5.0 to 0.004 mg mL -1 , while 0.0008 mg mL -1 and the control resulted in only 5% of mortality. In the topic application 60 to 65% of mortality were observed with dilutions of 25.0 to 0.1 mg mL -1 . Dilutions of 0.0008 to 0.2 mg mL -1 did not differ from the control. The repellence index was lower than the minimum value praised in literature to consider a substance as repellent. These results pointed out to the high potential of P. hispidum extract for the control of H. hampei. Key words -Coffea arabica, natural insecticide, Piperaceae RESUMO -(Atividade inseticida do extrato das folhas de Piper hispidum (Piperaceae) sobre a broca-do-café (Hypothenemus hampei)). Este trabalho teve como objetivo avaliar o potencial inseticida do extrato acetônico das folhas de Piper hispidumKunth sobre Hypothenemus hampei (Ferrari, 1867) em aplicação tópica, superfície contaminada e efeito de repelência. Adotou-se as diluições de 25,0; 5,0; 0,1; 0,02; 0,004 e 0,0008 mg mL -1 para exposição em superfície contaminada e aplicação tópica. No teste de repelência, utilizou-se a diluição 0,5 mg mL -1 do extrato. As avaliações foram realizadas nas 48 horas após a exposição ao extrato. Na exposição em superfície contaminada, obteve-se 100% de mortalidade, na diluição 25,0 mg mL -1 , e de 50 a 80% nas diluições 0,004 a 5,0 mg mL -1 , enquanto 0,0008 mg mL -1 e o controle resultaram em apenas 5% de mortalidade. Na aplicação tópica, atingiu-se 60 a 65% de mortalidade, com as diluições de 0,1 a 25,0 mg mL -1 . As diluições de 0,2 a 0,0008 mg mL -1 não diferiram do controle. Os índices de repelência foram inferiores ao valor mínimo preconizado na literatura para se considerar uma substância como repelente. Este estudo evidencia a atividade inseticida do extrato de folhas de P. hispidum sobre H. hampei, o que sugere seu potencial no controle deste inseto.Palavras-chave -Coffea arabica, inseticida natural, Piperaceae 1.

Field sampling was carried out in Ouro Preto d'Oeste - Rondônia (10 degrees 45'S and 62 degrees 15'W) to evaluate the mycobiota associated with Hypothenemus hampei Ferrari [cuticle, mouth, prothorax (mycangia), gut and feces] and its galleries on berries of Coffea canephora Pierre. Ten genera (201 isolates) were directly related with the insect while five genera (20 isolates) were related with galleries on berries. All the genera identified in the insects were also present in their galleries, what indicates that boring may be an active way of fungi inoculation by H. hampei. The fungi genera were more diverse in the mouth and prothorax of borers, and lower in feces. Fusarium, Penicillium and Geotrichum, with abundance of 55.7, 24.3 and 10.8%, respectively, were dominant genera. In the galleries Fusarium, Geotrichum, Trichoderma and Aspergillus with abundance of 33.3, 29.6, 18.5 and 14.8%, respectively, were dominant genera. The overall presence of Fusarium in coffee berry borer and its galleries) reinforces previous indications of a close interaction between H. hampei-Fusarium. The presence of Aspergillus and Penicillium emphasizes the possibility of "ochratoxin dispersion" by the borer. This work provides the first record of the mycobiota associated with H. hampei in C. canephora. Among the identified genera, Cephalosporium, Geotrichum and Oidiodendrum were recorded for the first time in association with H. hampei and its galleries in C. canephora.

RESUMO:O presente trabalho teve como objetivo investigar a composição química e avaliar a atividade inseticida do extrato acetônico das raízes de Piper alatabaccum sobre Hypothenemus hampei. As raízes foram coletadas no campus da Universidade Federal de Rondônia, em Porto Velho-RO. Diluições de 100,0, 50,0, 25,0, 5,0 e 0,5 mg mL -1 foram utilizadas para exposição na superfície contaminada e aplicação tópica. No teste de repelência, utilizou-se as diluições de 25,0 e 100,0 mg mL -1 . As avaliações ocorreram durante 48 horas após a exposição ao extrato. O extrato foi eficiente na mortalidade em aplicação tópica e superfície contaminada, não sendo eficiente no efeito de repelência. Estes resultados apontam para o elevado potencial inseticida do extrato de P. alatabaccum no controle de H. hampei. Identificou-se cinco compostos, sendo três amidas, dihydropiplartine piperovatina e piplartina, e duas flavona, 3 ',4',5,5',7 pentamethoxyflavone e 5,5',7-trimetóxi-3',4'-methylenedioxyflavone. Palavras-chave:Coffea arabica, Piperaceae, broca-do-café, repelência. ABSTRACT: Chemical constituents and insecticidal activity of the crude acetonic extract of Piper alatabaccum Trel & Yuncker (Piperaceae) on Hypothenemus hampei Ferrari.The purpose of this work was to investigate the chemical composition and to evaluate the insecticidal activity of the acetonic extract of Piper alatabaccum roots on Hypothenemus hampei. Roots of P. alatabaccum were collected at the University of Rondônia in Porto Velho-RO. Dilutions of 100.0, 50.0, 25.0, 5.0 and 0.5 mg mL -1 were used for exposition in the contaminated surface and for topical application. For the repellence test, 25.0 and 100.0 mg mL -1 dilutions were used. The evaluations occurred during 48 hours after exposure to the extract. The extract was efficient in terms of mortality, but no repellence was observed in the bioassays. These results pointed out the high insecticidal potential of the P. alatabaccum extract to control H. hampei. Five compounds, three amids, piperovatine, dihydropiplartine and piplartine, and two flavones, 3 ',4',5,5',7-pentamethoxyflavone and 5,5',7-trimethoxy-3',4' methylenedioxyflavone were identified.

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