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The objective of this study was to evaluate the flow of a film of bio-oil in a cylindrical tube similar to the tube of a heat exchanger found in a fast pyrolysis unit of sisal residue in a fluidized bed. The velocity profile of bio-oil was determined from the momentum balance and the equation that describes the rheological behavior of the fluid. The rheological behavior of bio-oil was determined at 60, 70, 80, 90, and 110 °C, and eight models to describe the rheological behavior were evaluated. The Sisko, Herschel–Bulkley, Mizrahi−Berk and Robertson−Stiff models all performed similarly, and they adequately represented the rheological behavior of bio-oil with a pour point of 55 °C derived from sisal residue. The variation of the thickness of the bio-oil film on the walls of a cylindrical tube and the velocity profile in the film as a function of temperature were determined empirically using the four best rheological models. Only the equation of flow employing the rheological model of Sisko showed results consistent with the behavior of the fluid.