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Herein, an attempt has been made to synthesize silver nanoparticles from Murraya koenigii leaf extract at room temperature. The synthesized materials are stable for several months. The UV‐vis spectrophotometric analysis is carried out to ensure the formation of silver nanomaterials. XRD pattern confirms the formation of polycrystalline silver nanomaterials with the cubic crystal structure. Irregularly shaped particles are observed under field emission scanning electron microscopic observations. Elemental studies show the presence of Ag and O elements. The synthesized nanomaterial is multi‐applicative and shows potential efficacy against pathogenic microorganisms like Pseudomonas aeruginasa and Staphylococcus aureus. The results show that the leaf extract of M. koenigii is an efficient bio‐reductant for the synthesis of silver nanomaterials. The synthesized nanoparticles show potent anti‐microbial activities against Gram‐positive and Gram‐negative bacteria under study.