In the present study, induced breeding of endemic barb, Dawkinsia filamentosa was carried out using three different synthetic hormones namely Human chorionic gonadotropin (HCG), WOVA-FH and Ovatide at different (0.3, 0.5, 0.7 and 0.9 ml/Kg) doses. All synthetic hormones showed good performance at 0.7 ml/kg body weight. Comparatively WOVA-FH showed better performance with higher number of spawned eggs (1405±5.74 nos/female) and hatching rate (90±0.6%). The study concluded that WOVA-FH at 0.7 ml/kg is an ideal for induced breeding of D. filamentosa. Fertilised eggs were golden in colour with an orange tinge. Hatching took place 34- 36 hrs (26±0.50C) of post fertilization. Yolk sac remained up to 60 hrs of post hatching. Complete developmental stages of D. filamentosa from egg to adult were recorded in captive condition. The present study aids in introducing hatchery-bred seeds of valuable indigenous fish Dawkinsia filamentosa into the ornamental fish trade. Availability of hatchery seed may reduce the wild exploitation and contributes to the conservation of the natural resources.

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Cryopreservation of fish gametes is an emerging technology and breeding with cryopreserved gametes is advancement in fish seed production. Success of cryopreservation is evaluated by the post -thaw motility of the spermatozoa, an for which energy is required. Cryopreservation is known to cause changes in the seminal plasma that would alter the energy supply for the motility of the spermatozoa. Therefore, energy supplementation is found to be useful during cryopreservation. Cirrhinus mrigala spermatozoa were cryopreserved along with glucose as a co-cryoprotectant after 1:100 dilutions with 0.85% physiological saline as extender and Dimethyl Sulfoxide (DMSO) as cryoprotectant (85:15). The diluents contained glucose at four different concentrations, viz., T 1 (0.25%), T 2 (0.5%), T 3 (0.75%) and T 4 (1%). The diluted milt was equilibrated for 10 min at 5˚ C and loaded into 0.25 ml straws. The loaded straws were then frozen with LN 2 vapour for 5 min and immersed in liquid nitrogen. Observations were made once in 7 days for 42 days on motility parameters based on which the duration, score, pattern and percentage were determined. The spermatozoa cryopreserved with glucose at 0.5% concentration showed the highest motility duration of 204±3.6 s whereas Control group showed motility duration of only 83.33± 4.5 s on 42 nd day. The difference in motility duration was statistically significant (P>0.025). The present study revealed the benefits of adding glucose at 0.5% during cryopreservation as it could help in maintaining the motility duration and survival of spermatozoa.