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COMBINATIONS OF INSULIN, SELENIUM AND TRANSFERRIN THAT MAXIMIZE MATURATION, FERTILIZATION AND CULTURE 2.1 INTRODUCTION 2.1.1 The selection of a buD for in vitro fertilization 2.1.2 Addition of insulin-transferrin-selenium (ITS) to maturation medium 2.1.3 The addition of insulin, selenium or transferrin individually or in combination to maturation, fertilization or culture medium 2.2 MATERIALS AND METHODS 2.2.1 Oocyte recovery and selection 2.2.2 In vitro maturation procedure 2.2.3 Maturation controls 2.2.4 Assessment of sperm motility and morphology 2.2.5 In vitro fertilization procedure 2.2.6 Fertilization controls 2.2.7 In vitro culture procedure 2.2.8 Experimental design and statistical analysis 2.3 RESULTS 2.3.1 Bull selection 2.3.2 Addition of ITS to maturation medium 2.3.3 Insulin, selenium and transferrin in the maturation, fertilization and culture medium 31 33