In this study, experimental tests were performed to evaluate the shear performance of encased steel truss (EST) composite girders that can resist loads at construction and composite stages. Four full-scale EST composite girders were fabricated, where the truss type (Pratt truss and Warren truss) and presence of stirrups were set as main test variables. The test results showed that in specimens applied with the Pratt truss, horizontal shear cracking occurring along the interface between concrete and steel was the dominant failure mode. Based on the crack pattern and failure plane observed from the test, the horizontal shear strengths of the Pratt truss specimens were calculated, which provided conservative results. On the other hand, in the specimens with the Warren truss inside, the strengths of the specimens were governed by the shear failure occurring in the screw rod connecting the truss elements prior to the yielding of the diagonal member. The shear strengths of the Warren truss specimens calculated based on the shear failure of the screw rod were similar to that obtained from the test.