The goal of this study was to assess the antimicrobial activity of the essential oil and aqueous, alcoholic, and ethyl acetate extracts of Guarea kunthiana A. Juss against ten Salmonella serotypes from poultry origin (Enteritidis, Infantis, Typhimurium, Heidelberg, Mbandaka, Give, Saintpaul, Ohio, Gallinarum, and Agona); the insecticidal potential of the oil and extracts against larvae and adults of Alphitobius diaperinus (Coleoptera: Tenebrionidae) (Panzer, 1797); and also to determine the antioxidant activity of these compounds using the capture method of radical 2.2-diphenyl-2-picrylhydrazyl (DPPH). With respect to the antimicrobial activity of the essential oil of G. kunthiana, the most sensitive serotypes were Infantis, Typhimurium, and Give, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 54.6 µg/ml. Regarding the other serotypes tested, the action of the oil was classified as moderate, weak, or inactive. With respect to the extracts, the greatest microbial susceptibility was observed in the activity of the alcoholic extract, with MIC and MBC values of 0.39 mg/ml for the serotype Infantis, and MIC and MBC values of 0.78 mg/ml for the serotype Gallinarum. The results of the insecticidal activity of the essential oil and the extracts were found to be low, with 28% mortality of larvae and 12% of adults, at a concentration of 200 mg/ml. Regarding the extracts, the best results were observed using the alcoholic extract at concentrations of 10%, with 34 and 44% mortality of larvae and adults, respectively. The values of antioxidant activity showed that there were no significant differences between the synthetic antioxidant butylhydroxytoluene (BHT), the essential oil, and the alcoholic extract, revealing that both the essential oil and the alcoholic extract of G. kunthiana exhibited high antioxidant capacity.

Efeito de defensivos agrícolas naturais e extratos vegetais sobre parâmetros biológicos de AbstractThis study was carried out aiming to evaluate the effect and compatibility of vegetal and Pycnoporus sanguineus basidiocarps extracts and alternative products on biological parameters of Metarhizium anisopliae fungus. Extracts (solution in water 10%) and natural products (AR = average field recommendation; 0.5 AR and 2.0 AR) are applied on PDA culture media surface previously inoculated with fungi conidia. The effect of the treatment on conidia germination, vegetative growth and conidiogenesis was compared. Most alternative products were compatible to the fungus, and only Bordeaux mixture AR and 2.0 AR were moderately toxic to M. anisopliae. Although some significativally negative effect there were observed on conidial viability (reduction of 50 to 80% by alcoholic extracts), all extracts were compatible. This point to the necessity to be careful with application, avoiding mixtures or subsequent use of products less than 48 hours after fungi application.

This study was performed under laboratory conditions to identify isolates of the fungus Beauveria spp. that can control Polyphagotarsonemus latus in the greenhouse and field. Thirty Beauveria spp. isolates were tested by spraying 1 mL conidia (1 × 10 8 conidia/mL) on pepper leaf discs containing 15 mites. Evaluations were performed on the 3rd and 6th day post application by counting the number of dead mites. Vegetative growth and conidial production were measured from the selected isolates, and bioassays were conducted in the greenhouse on bean seedlings in plastic pots. The isolate Unioeste 53 was selected, and a conidial suspension (1 × 10 8 conidia/mL) was applied with a backpack sprayer. The evaluation consisted of pre-and post-treatment counts of the number of live mites on ten leaflets in both the plots treated with the fungus and control plots, and the same procedure was followed for the field experiment. In the laboratory, the Unioeste 53 isolate resulted in total and confirmed mortality rates of 70% and 57.7%, respectively. In the greenhouse, the population decreased by 76.71% by the 16th day after application. In the field, the population decreased by 66% by the 12th day after application, demonstrating the potential of this fungus for mite management.Keywords: microbial control, entomopathogenic fungi, broad mite. Seleção e caracterização de isolados do fungo ResumoEste trabalho teve por objetivo selecionar isolados do fungo Beauveria bassiana em condições de laboratório com potencial de uso em casa de vegetação e campo no controle do ácaro branco. Foi realizada uma seleção com 30 isolados de Beauveria spp. através de pulverização direta de conídios (1 × 10 8 conídios/mL) sobre discos foliares de pimenta contendo 15 ácaros. As avaliações foram realizadas no terceiro e sexto dia contando-se o número de mortos, e confirmação do patógeno em câmara úmida. Parâmetros de crescimento vegetativo e produção de conídios foram avaliados. No bioensaio em casa de vegetação foram preparados vasos com plantas de feijão e fez-se a aplicação do Unioeste 53 (1 × 10 8 conídios/mL), utilizando pulverizador costal, a avaliação constou da contagem prévia e posterior à aplicação do número de ácaros vivos em 10 folíolos, tanto nas parcelas destinadas ao tratamento com fungo, quanto na testemunha. A fase de campo seguiu os mesmos padrões, porém, com área experimental total de 225 m 2 , com oito parcelas de 10,24 m 2 , sendo 4 testemunhas e 4 onde foi aplicado o isolado Unioeste 53 seguindo metodologia de aplicação e avaliação já descritas para casa de vegetação. Em laboratório o isolado Unioeste 53 causou mortalidade total de 70% e 57,7% de mortalidade confirmada. Em casa de vegetação, apresentou redução da população de 76,71% 16 dias após aplicação, já em campo, a redução da população foi de 66% após 12 dias da aplicação, demonstrando o potencial do ácaro pelo fungo.Palavras-chave: controle microbiano, fungos entomopatogênicos, ácaro branco.

The fungus Beauveria bassiana is naturally found in poultry houses and causes high rates of mortality in Alphitobius diaperinus. Laboratory and field experiments have shown the potential of this fungus as an insect control agent. However, in poultry houses, bacteria as Salmonella, can be found and have been studied alternative control methods for this pathogen. Thus, this study aimed to evaluate the effect of plant extracts and a disinfectant on the fungus Beauveria bassiana (strain Unioeste 4). Conidial viability, colony-forming unit (CFU) counts, vegetative growth, conidia production, insecticidal activity of the fungus and compatibility were used as parameters in the evaluation of the effect of these products on the fungus. Alcoholic and aqueous extracts of jabuticaba (Myrciaria cauliflora (Mart.), guava (Psidium guajava (L.)), and jambolan (Syzygium cumini (L.), at concentrations of 10% as well as the commercial disinfectant, Peroxitane  1512 AL, were evaluated at the recommended concentrations (RC), 1:200 (RC), 0.5 RC and 2 RC. There was a negative influence of alcoholic and aqueous extracts of jabuticaba, guava and three dilutions of Peroxitane on the viability of conidia. The CFUs and vegetative growth of the fungus were affected only by the Peroxitane (all dilutions). For conidial production, the aqueous extract of guava had a positive effect, increasing production, while the Peroxitane at the R and RC concentrations resulted in a negative influence. The mortality of A. diaperinus, caused by the fungus after exposure to these products, was 60% for the peracetic acid at 0.5 RC, and above 80% for the extracts. Thus, the results showed that all the extracts and Peroxitane at RC 0.5 are compatible with the fungus B. bassiana Unioeste 4, however only the extracts had a low impact on inoculum potential. Keywords: Myrciaria cauliflora), na concentração de 10% e também o desinfetante comercial Peroxitane  1512 AL na concentração recomendada -1:200 (CR), 0,5 CR e 2CR. Foram avaliados a: germinação dos conídios, unidades formadoras de colônias (UFC), crescimento vegetativo, produção de conídios e efeito sobre a atividade inseticida do fungo contra adultos de A. diaperinus, bem como a compatibilidade entre produtos e o fungo. Verificou-se influência negativa dos extratos alcoólico e aquoso de jabuticabeira, goiabeira e das três diluições de Peroxitane sobre a viabilidade dos conídios. Já, a UFC e o crescimento vegetativo foram afetados apenas com Peroxitane (em todas as diluições). Para produção de conídios, o extrato aquoso de goiabeira teve efeito positivo, elevando a produção, enquanto as diluições recomendada e o dobro de Peroxitane mostraram influência negativa. Observou-se ainda que a mortalidade de A. diaperinus causada pelo fungo após a exposição aos produtos foi de 60% para o ácido peracético na 0,5 CR, já para os extratos foi acima de 80%. Assim, os resultados demonstraram que todos os extratos e o Peroxitane na 0,5 CR são compatíveis com o fungo B. bassiana Unioeste 4, porém apenas os extratos...

No abstract