The application of DNA barcoding represents a complementary and efficient approach to identifying specimens at all stages of their life cycle when used in combination with traditional morphological methods. Due to difficulties obtaining samples from the deep sea (> 200 m), these methods have been less frequently applied to deep-water taxa. We used DNA-barcoding techniques to enhance large-scale biodiversity initiatives for deep-pelagic crustaceans within the Gulf of Mexico, a region that has recently been identified as one of the world’s four most hyperdiverse ocean ecosystems. This study was conceptualized in direct response to the Deepwater Horizon Oil Spill in 2010, which identified major knowledge gaps in our understanding of deep-sea biodiversity. We employed traditional Sanger sequencing and a genomic skimming approach to target the mitochondrial ribosomal large subunit 16S and the protein-coding cytochrome oxidase subunit 1 (COI). Alongside these molecular approaches, traditional taxonomic investigations allowed for advancements in biodiversity, evolutionary relationships, cryptic species complexes, and distributional records across four abundant and common deep-pelagic orders (Amphipoda, Euphausiacea, Lophogastrida, and Decapoda). DNA barcodes were successfully obtained from 82 species for a total of 158 and 169 new 16S and COI sequences, respectively. Evidence of cryptic diversity has been found in the genera EucopiaDana, 1852 (Lophogastrida) and Allosergestes Judkins & Kensley, 2008 (Decapoda). New records for the Gulf of Mexico of species of LanceolaSay, 1818 (Amphipoda), Eupasiphae Wood-Mason in Wood-Mason & Alcock, 1893, PasiphaeaSavigny, 1816, and MeningodoraSmith, 1882 (Caridea) are presented. Preliminary results allow us to reconsider the current classification and evolutionary relationships of several lineages. The urgency to document biodiversity in the deep-pelagic is pressing against a backdrop of future threats including oil spills and deep-sea drilling.

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Deep-sea shrimp of the family Sergestidae Dana, 1852 provide a unique system for studying the evolution of bioluminescence. Most species within the family possess autogenic bioluminescent photophores in one of three distinct forms: lensed photophores; non-lensed photophores; or internal organs of Pesta. This morphological diversity across the Sergestidae has resulted in recent major taxonomic revisions, dividing the two major genera (Sergia Stimpson, 1860 and Sergestes Milne Edwards, 1830) into 15. The present study capitalises on molecular data to construct an updated genus-level phylogeny of sergestid shrimp. DNA was successfully extracted from ~87 individuals belonging to 13 of the 15 newly proposed genera. A ‘genome skimming’ approach was implemented, allowing the capture of mitochondrial genomic data across 19 sergestid species. Additional individuals have been incorporated into the phylogeny through Sanger sequencing of both nuclear (H3 and NAK) and mitochondrial (16S and COI) genes. The resulting molecular phylogeny is compared with previous morphological trees with specific attention to genus-level relationships. The -sergestes group was rendered non-monophyletic and the -sergia group was recovered as monophyletic. Ancestral state reconstructions of light organ type indicate that organs of Pesta is the ancestral state for the family. Non-lensed photophores evolved once across the -sergia group, but were later lost in the deepest living genus, Sergia. Lensed photophores also evolved once within the genera Prehensilosergia Vereshchaka, Olesen & Lunina, 2014, Lucensosergia Vereshchaka, Olesen & Lunina, 2014 and Challengerosergia Vereshchaka, Olesen & Lunina, 2014. These findings identify preliminary patterns across light organ type and species’ depth distributions; however, future research that incorporates finer-scale depth data and more species is needed to confirm our findings.

We have compared the convenience and accuracy of two models using 6 shell dimensions for predicting 4 biomass parameters for 4 species of southern U.S.A. unionids (Quadrula quadrula, Q. pustulosa, Lampsilis anodontoides, and Amblemaperplicata).Prediction of whole wet weight, tissue wet weight, tissue dry weight, and shell dry weight as a linear function of (shell length)3 was accurate, even with extremely small sample sizes. In addition, this method is very convenient for field use because it requires one simple, unambiguous shell measurement.

Relict glacial and periglacial environments are widespread, and the deposits that they are associated with mean it is inevitable that the design and construction of many projects will be influenced by their presence and nature. Tills and other glaciogenic deposits prove to be particularly challenging in this context for reasons that include: the spatial variability of the nature of the deposits; the wide range of particle sizes often included within a given soil, including large-sized particles; spatial variation in soil type and properties; variation in depth to rockhead and variable degrees of weathering and alteration; the presence of groundwater, that is misinterpreted as perched water, as well as sub-artesian and artesian conditions; the presence of solution features and fissures, partly or completely infilled with soft or loose material; and the presence of (often shallow) shear surfaces at residual strength. In this chapter, some of the more common problems and associated solutions associated with earthworks and man-made slopes, tunnels and underground structures, dams and reservoirs, foundations, and offshore engineering and installations are reviewed. It is important that great care is taken in addressing the influences of variability, complexity and uncertainty inherent in glacial/periglacial soil formations at all stages of the construction process, from feasibility to end-of-project activities, such as preparation of the as-built drawings.