Girardinia heterophylla, known as Himalayan Nettle commonly grows in temperate and subtropical Himalayas. Plant leaves were collected in winter season and analyzed using appropriate chemical methods and the biochemical constituents were ascertained. An unknown compound was isolated from leaves of the plant. After being collected, analyzed by GC-MS and compared with NIST standard chart library, it was declared to be -sitosterol, an epimer of -sitosterol.sitosterol has been reported for the first time in Girardinia heterophylla and has potential to be used as an antidiabetic owing to its remarkable medicinal properties.

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Girardinia heterophylla (Family: Urticaceae) roots has not been studied so far. The swollen base of roots were collected from and extracted with petroleum ether. The dried petroleum extract was subjected to column chromatography and TLC. Three compounds were isolated from the roots of Girardinia heterophylla. On the basis of spectral analysis they were identified as β-sitosterol, γ-sitosterol and ursolic acid. In this study the presence of γ-sitosterol and Ursolic acid in roots of Girardinia heterophylla has been reported for the first time.

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