One of the critical steps for the commercialization of new enzymebased products is the successful scaleup of the catalyzed reaction. In the study presented here, we achieved a scaleup for the enzymatic production of glycerol adipate on a 500 g scale in a heated, solvent-free system. The influence of various reaction conditions (i.e., temperature, pressure, enzyme concentration, reactants ratio, stirrer type, stirring rate, and reaction time) on the substrate conversion and molecular weight of the product was investigated. Conversions were higher than 0.9, and molecular weights were in the desired range of 2000-3000 Da. Space time yields of 370 g d -1 L -1 could be achieved. Maximal polymer yield was achieved at 60 °C, < 20 mbar, 3 wt % Novozym 435, glycerol:adipic acid ratio 1.1:1, <48 h while stirring at 100 rpm.