Detailed pathomorphological study was carried out on tumor suspected mesenteric lymphnode from a Jersey cross bred cow. Uniform population of small non cleaved cells with prominent nucleoli were observed in cytological study, histopathology revealed characteristic starry sky appearence or moth eaten appearance of lymph node with diffuse infiltration of neoplastic cells in cortex and medulla. Immunohistochemistry shown positive reaction for common acute lymphocytic leukemia antigen (CD10), terminal deoxynucleotidyl transferase (TdT) and cluster of differentiation 5 (CD5) and negative reaction for cluster of differentiation 3 (CD3), B-cell lymphoma 2 (Bcl2), cluster of differentiation 20 (CD20) and cluster of differentiation 79a (CD79a). These Cytological, histopathological and immunohistochemical findings observed led to final diagnosis of burkitt lymphoma.