West African okra occurs in wild and unselected variants in Nigeria but farmers desire stable and high-yielding cultivars. Twenty-five West African okra genotypes from diverse geographical backgrounds were evaluated in five different environments for stability of performance. Performance was measured by number of days to 50% flowering, number of pods per plants, number of seeds per pod, plant height at maturity and seed yield per plant. A regression method, Additive main effects and Multiplicative Interaction (AMMI) and Genotype main effect and genotype x environment Interaction (GGE) were employed in the evaluation. Joint regression and AMMI analyses showed significant (P< 0.01) G x E interaction with respect to seed yield, and both identified NGAE-96-0060 and NGAE-96-0063 as stable genotypes. The AMMI and GGE biplot analyses are more efficient than the Eberhart and Russell analysis. The GGE biplot explains higher proportions of the sum of squares of the GxE interaction and is more informative with regards to environments and cultivar performance than the AMMI analysis. GGE-biplot models showed that the five environments used for the study belonged to three mega-environments with environment 2 (Upland, 2007) being the most representative and most desirable of all. The GGE results also confirmed NGAE-96-0063 as being stable with NGAE-96-04 as the most stable. NGAE-96-04 was identified as most superior genotype in terms of yield and stability of performance and could be recommended for cultivation.
The identification of suitable parental genotypes potentially generating superior lines with traits contributing to the overall yield of cowpea is an important step in the development of improved varieties. Eight parents and their 28 hybrids obtained through diallel crossing design were evaluated in a randomized complete block design with three replications. Highly significant differences among the parents and their hybrids for different traits were observed. General combining ability (GCA) mean squares were larger than the specific combining ability mean squares for all the traits analysed. The ratio h2/H2 revealed different number of genes controlling the traits, pointing out their polygenic inherited control. The estimated low narrow sense heritability recorded in this study indicated that recurrent selection procedure could be effective to improve the analysed traits in the genotypes included in this study. Kananado with large, positive and significant GCA effects was identified as the parent with desirable genes for the genetic improvement of the considered yield component in cowpea. In addition, IT03K-316-1 x Cowpea-2 and IT99K-316-2 x Kananado seemed to be the best hybrids for number of pods/plant and 100-seed weight, respectively
The success of a hybrid breeding program is dependent on available heterotic patterns for exploitation of grain-yield heterosis. The efficiency of the assignment of germplasm lines into heterotic groups is a prerequisite for obtaining useful heterotic patterns among germplasm lines. A total of 256 maize hybrids, comprising 244 top crosses, six diallel cross hybrids, and six checks, were grown under Striga infestation, drought, and optimal conditions, from 2015 to 2017. The study determined the combining abilities of the parental inbreds, classified the inbreds into heterotic groups, and compared the efficiencies of the following four grouping methods for classifying the inbreds: specific combining ability (SCA) effect of grain yield; general combining ability (GCA) effects of multiple traits (HGCAMT); SCA and GCA (HSGCA) for yield; and single nucleotide polymorphism-based genetic distance (SNP-based genetic distance (GD)). Significant GCA and/or SCA mean squares were revealed for most measured traits in all test environments. Sums of squares (SS) due to GCA were higher than SCA SS for measured traits in all test environments. The HSGCA, SCA, and SNP-based GD methods identified four heterotic groups, whereas the HGCAMT identified three groups, in all environments. The additive gene effect was preponderant in the inheritance of most measured traits. The efficiencies of the grouping methods varied with the test environments. The HSGCA and SCA methods were the most efficient for grouping in all test conditions. For practical breeding purposes, the HGCAMT and HSGCA methods were recommended under Striga infestation and drought, respectively. The heterotic patterns, which were revealed in this study, were effective for planning hybridization schemes for developing high-yielding, Striga-tolerant/resistant, and drought-tolerant maize hybrids for stressful environments.
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