Tuberculosis (TB) and human immunodeficiency virus (HIV) are major devastating infectious diseases African countries and other regions of the world. TB is a potentially serious infectious bacterial disease that mainly affects the lungs. While HIV is a virus that attacks the body's immune system; which if left untreated, can lead to acquired immunodeficiency syndrome (AIDS). Thus, this study was carried out to monitor the prevalence of tuberculosis and human immunodeficiency virus (HIV) situation in Ikere-Ekiti, Ekiti State Southwestern Nigeria. A total of 191 participants were enrolled in this study. Blood and sputum samples were used in screening of HIV and Mycobacterium tuberculosis respectively. 102(53.4%) participants were confirmed HIV positive cases and 89(46.6%) participants were confirmed as M. tuberculosis-positive cases. The rate of tuberculosis was higher among women than among men and prevalence of HIV was slightly higher among men than among female. The study shows that age group 26 – 30 years have the highest rate of tuberculosis and age group 36 – 40 years have the highest rate of HIV cases while the highest number of TB with HIV co-infection 34 (17.8%) were gotten from the age group 36 – 40years. The rate at which the prevalence of tuberculosis, HIV and their co-infection are rising is due to various challenges facing its eradication. Adequate resource mobilization and effective spending is needed to achieve success. In addition, efforts should be made to improve the surveillance system.