Proteins were isolated from chickpea flour by micellization and isoelectric precipitation techniques. Protein content ranged from 84.8-87.8%. Denaturation temperature and transition enthalpy, by differential scanning calorimetry, were higher for micelle than for isoelectric proteins. Sodium dodecyl sulfate-polyactylamide gel electrophoresis showed a molecular weight distribution between 16.6-66.4 kD for micelle and 14.9-84.2 kD for isoelectric proteins. Most functional properties compared favorably to a commercial soy isolate. In general, most essential amino acids of chickpea isolates were at acceptable levels compared to a reference pattern. High values of in vitro digestibility and calculated PER were obtained for the isolates.

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The effects of calcium hydroxide and screw speed on expansion, pasting, crystallinity, torque, water absorption, and solubility indices upon extruded blue maize meal products were studied, using a central composite experimental design. All the characteristics measured presented a significant quadratic regression model, indicating relationship between the responses and extrusion conditions. It was found that the expansion, crystallinity, torque, water absorption, and solubility indices decreased and the Rapid Viscosity Analyzer's (RVA) pasting characteristics increased with the increase of calcium hydroxide concentrations. The Pearson correlation indicated that the responses presented strong correlation (r > 0.7, p < 0.01) between them. Extruded products with physical appearance and expansion similar to the control were found at concentrations lower than 0.1% of calcium hydroxide.

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