Foxtail millet (Setaria italica (L.) P. Beauv.) is an ideal crop for changing climate and food habits of peoples due to its short duration, high photosynthetic efficiency, nutritional richness and fair resistance to pest and diseases. However, foxtail millet yields are low mainly due to the lack of effort for its improvement and the lack of proper utilization of existing genetic variability. To enhance the use of diverse germplasm in breeding programmes, a core collection in foxtail millet consisting of 155 accessions was established. Core collection accessions were fingerprinted using 84 markers (81 simple sequence repeats (SSRs) and three Expressed Sequence Tag (EST)-SSRs). Our results showed the presence of greater molecular diversity in the foxtail millet core collection. The 84 markers detected a total of 1356 alleles with an average of 16.14 alleles (4 -35) per locus. Of these, 368 were rare alleles, 906 common alleles and 82 the most frequent alleles. Sixty-one unique alleles that were specific to a particular accession and useful for germplasm identification were also detected. In this study, the genetic diversity of foxtail millet was fairly correlated well with racial classification, and the race Indica showed a greater genetic distance from the races Maxima and Moharia. The pairwise estimate of dissimilarity was . 0.50 except in 123 out of 11,935 pairs which indicated a greater genetic variability. Two hundred and fifty pairs of genetically most diverse accessions were identified. This large molecular variation observed in the core collection could be utilized effectively by breeders or researchers for the selection of diverse parents for breeding cultivars and the development of mapping populations.
Use of diverse germplasm is a key factor which allows high level of resolution due to extensive recombination in the history. Therefore, population used in association mapping should posses as many phenotypes as possible. One of the methods to obtain most of the phenotypes is to construct the core collection. The ICRISAT foxtail millet core collection consisting of 155 accessions was genotyped using 72 simple sequence repeat (SSR) markers to investigate the genetic diversity, population structure and linkage disequilibrium (LD). A high degree of molecular diversity among the accessions was found, with an average of 16.69 alleles per locus. STRUCTURE analyses classify the accessions into four subpopulations (SP) based on SSR allelic diversity. The Neighbor joining clustering and the principal coordinate analysis were in accordance with the racial classification. The distribution of molecular genetic variation among and within the four SP and three races showed high degree of variability within each group, and low level of genetic distance (GD) among the groups. LD decay of \40 cM of GD in foxtail millet core collection was observed, which suggests that it could be possible to achieve resolution down to the 40 cM level. From this investigation, it is evident that the foxtail millet core collection developed at ICRI-SAT is very diverse and could be a valuable resource for trait association mapping, crop breeding and germplasm management.
Twelve quantitative characters were taken under observation to estimate substantial variation and relationship among Guar (Cyamopsis tetragonoloba (L.)Taub.,Fam.: Fabaceae) genotypes and to identify the best performing lines suited to southern parts of India, where the potential of growing the guar crop throughout the year. Analysis of variation for such quantitative traits in diverse line showed considerable and dissimilar level of variability. The largest variation was found for days to maturity, plant height, pods per plant and cluster per plant. Single plant yield was significant positively correlated with primary branches per plant, secondary branch per plant, cluster per plant, pods per cluster, pods per plant, however pod length was significant but negatively correlated with single plant yield. Principal component analysis showed the amount of variation by principal components 1 to 5 viz., 38, 16.4, 12.5, 9.5 and 6.6%, respectively. Clustering analysis based on various morphological traits assorted 42 guar genotypes into four main groups and six subclusters. Dendrogram based on hierarchal clustering grouped genotypes based on their morphological traits rather than geographic origin. Grouping of genotypes based on morphological traits is not always associated with their geographical origin. Out performed genotypes based higher yield potential, 100-seed weight, pods per plant and early maturity are identified for guar variety development and future breeding programme suited for South India. DOI: http://dx.doi.org/10.3329/bjb.v44i1.22724 Bangladesh J. Bot. 44(1): 59-65, 2015 (March)
In the past, very little efforts have been taken for development of inbred lines of brinjal through the exploitation of genetic variability present in the exotic hybrids. F2 generation obtained from the selfing of F1 hybrid provides all possible variations. So, selection with particular objectives in F2 generation is very much effective and selfing of those selected genotypes generation after generation helps to develop inbred lines (similar to the parental lines of the exotic hybrids). These inbreds with desired characters including high yield potential can be used as High Yielding Variety (HYV) as well as the parents for hybrid variety. To increase the genetic yield potential, the maximum utilization of the desirable characters for synthesizing of any ideal genotypes is essential. Variability in brinjal is expected to be immense as the fruits vary greatly in shape and size. The present investigation was undertaken at Department of Horticulture, Agricultural College and Research Institute, Madurai during 2011 to determine variability in segregants of eggplant (Solanum melogena L.). The crosses L5 x T4 (Palamedu Local x EP 65) and L4 x T1 (Alagarkovil Local x Annamalai) had the highest mean with high variability for individual fruit weight and fruit yield per plant. These crosses were best for using as a base population for further improvement in fruit yield and fruit weight as they had high heritability and genetic advance. Favorable low mean with high variability occurred for days to first flowering (earliness) in the crosses L5 x T2 (Palamedu Local x KKM 1) and L4 x T2 (Alagarkovil Local x KKM 1). Direct selection may be executed considering these genotypes for selection towards the development of early in flowering and high yielding brinjal variety.
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