<b><i>Background:</i></b> COVID-19 is a respiratory disease transmitted through respiratory droplets and has a high transmission rate. There is still no effective and approved antiretroviral treatment or vaccine, so preventative measures remain the key to contain this pandemic. Portugal is now in phase 3.2 of the mitigation of COVID-19, with it being imperative to understand the health literacy of our country and prevent a lack of information through community empowerment. <b><i>Material and Methods:</i></b> A cross-sectional study with a sample from a primary care facility was conducted. We collected demographic and epidemiological data and carried out a questionnaire by phone call. Descriptive statistics and nonparametric tests were used to assess associations between the independent variables and the level of health literacy. The level of significance was set at <i>p</i> &#x3c; 0.05. <b><i>Results:</i></b> Our sample included 222 subjects (median age 62 years), mostly females (<i>n</i> = 131), undergraduates (<i>n</i> = 193), and with at least 1 risk factor for COVID-19 (<i>n</i> = 144). Overall, younger individuals, females, graduates and the nonrisk group appeared to have higher levels of health literacy, with some exceptions. <b><i>Conclusions:</i></b> We observed a well-informed population. However, it being a pandemic situation, our intention was to identify and correct outliers/misconceptions. This work allowed a perspective of the current state/pattern of health literacy as well as its possible predictors. Furthermore, it raised awareness of the relevance of specific communication approaches. Further studies to understand the predictors of health literacy are necessary.

Upon infection with Mycobacterium tuberculosis (Mtb) the host immune response might clear the bacteria, control its growth leading to latent tuberculosis (LTB), or fail to control its growth resulting in active TB (ATB). There is however no clear understanding of the features underlying a more or less effective response. Mtb glycolipids are abundant in the bacterial cell envelope and modulate the immune response to Mtb, but the patterns of response to glycolipids are still underexplored. To identify the CD45+ leukocyte activation landscape induced by Mtb glycolipids in peripheral blood of ATB and LTB, we performed a detailed assessment of the immune response of PBMCs to the Mtb glycolipids lipoarabinomannan (LAM) and its biosynthetic precursor phosphatidyl-inositol mannoside (PIM), and PPD. At 24 h and 5 days of stimulation, cell profiling and secretome analysis was done using mass cytometry and high-multiplex immunoassay. PIM mainly affected antigen-presenting cells to produce both proinflammatory (IL-2, IL-6, IL-17A, TNF-α and GM-CSF), and IL-4 and IL-10 cytokines, but not IFN-γ. LAM triggered a similar, albeit weaker, response. By contrast, PPD induced an increase in IFN-γ-producing cells. Moreover, PPD also led to increased numbers of IL-2, IL-6, IL-10, IL-17A, TNF-α and GrzB-producing cells. Treatment with an anti-TLR2 antibody led to partial inhibition of PIM-induced IL-6 production in myeloid cells, suggesting that PIM induces IL-6 production through TLR2. Expansion of monocyte subsets in response to PIM or LAM was reduced in both ATB and LTB as compared to healthy controls, suggesting a hyporesponsive/tolerance pattern in Mtb-infected individuals.