The roots of Hemidesmus indicus R.Br. was collected from Maruthamalai hills, Coimbatore and were extracted with 90% ethanol and fractionated by column chromatography using different solvents. A phytochemical Terpenoid was isolated using thin layer chromatography. The infrared spectrum was used for characterization of organic, inorganic and biological compounds. FT-NMR spectroscopy is used to determine the molecular structure based on the chemical environment of the magnetic nuclei like 1 H, 13 C, 31 P, etc., even at low concentrations. The carbon, hydrogen and oxygen content of the compound were analyzed by Erlinmeier flask method. Mass spectrometry has become a vital tool for giving qualitative and quantitative information on molecules based on their structural compositions.

Objective: Myocardial infarction (MI) continues to be a major public health problem in the world. The prepared polyherbal formulation (PHF) exhibit cardioprotective effects by several mechanisms. The objective of the research was to evaluate the antioxidant effect of a polyherbal formulation in isoprenaline hydrochloride (ISO) induced myocardial infarction in rats.Methods: Oxidative stress and myocardial infarction was induced by administration of isoprenaline hydrochloride (25 mg/kg) for 2 d. The degree of protection was determined by measuring the levels of lipid peroxidation (LPO), enzymatic antioxidants (superoxide dismutase-SOD, Catalase, Glutathione peroxidase) and non-enzymatic antioxidants (Vitamin C, E and reduced glutathione-GSH).Results: Two different doses (250 mg and 500 mg/kg body wt) of the formulation was used to prove the cardioprotective effect. Pretreatment with polyherbal formulation significantly (P<0.05) lowered LPO and elevated the enzymatic and non-enzymatic antioxidants in heart homogenate. Conclusion:The polyherbal formulation exhibited cardioprotective activity, which could be attributed to its antioxidant activity.