Objective: Isolate, screen and identify Streptomyces sp. from mangrove soil from pichavaram, Tamil Nadu, India, and study the molecular identification of selected Streptomyces sp. and check the antimalarial activity for the purified compound.Methods: The16SrRNA secondary structure and the restriction sites of KMA08 were predicted using Genbank online software, respectively. Antiplasmodial activity of the 80% Ethyl acetate extract of Streptomyces sp. against chloroquine-sensitive Plasmodium berghei in mice using the 4 days suppression test was conducted. A total of 30 mice assigned to 5 groups of 6 animals each were infected with chloroquine-sensitive (P. berghei) intraperitoneally. The Ethyl acetate extract (10, 20, and 30 ml/kg), standard drug (chloroquine, 10 mg/kg) and distilled water were administered orally daily for the treatment period. Percent Parasitemia was determined on the 5 th day from Giemsa stained smears obtained from tail vein and percent parasitemia suppression was calculated. Daily measurement of rectal temperature was also taken while body weight and packed cell volume were recorded on day 0 and 5. Results:Results showed the extract produced a dose-dependent reduction in parasite density compared to the control group. Percept parasitemia calculation revealed 21.3, 65.3, and 80.5% inhibition at 10, 20, and 30 ml/kg of the extract, respectively. Conclusion:The study revealed the present work indicated that Streptomyces sp. has as promising antiplasmodial activity against chloroquine sensitive P. berghei in a dose-dependent. As part of the drug discovery process, these promising finding may contribute to the medicinal and pharmaceutical field for malarial treatment.