The genus Acanthamoeba comprises free-living amebae identified as opportunistic pathogens of humans and other animal species. Morphological, biochemical and molecular approaches have shown wide genetic diversity within the genus. In an attempt to determine the genetic relatedness among isolates of Acanthamoeba we analyzed randomly amplified polymorphic DNA (RAPD) profiles of 11 Brazilian isolates from cases of human keratitis and 8 American type culture collection (ATCC) reference strains. We found that ATCC strains belonging to the same species present polymorphic RAPD profiles whereas strains of different species show very similar profiles. Although most Brazilian isolates could not be assigned with certainty to any of the reference species, they could be clustered according to pattern similarities. The results show that RAPD analysis is a useful tool for the rapid characterization of new isolates and the assessment of genetic relatedness of Acanthamoeba spp. A comparison between RAPD analyses and morphological characteristics of cyst stages is also discussed.
O estudo aponta a importância da memória nas indagações epistêmicas da antiga cultura grega. Inicialmente, fala sobre a presença da deusa Mnemosyne e suas lhas Musas no mito hesiódico e em seguida as estratégias mnemônicas para as competições e recitações poéticas. Depois, analisa as mudanças ocorridas com a introdução dos discursos filosóficos nos debates públicos e competições. Em seguida, aborda-se o lugar de Mnemosyne nos cultos órficos e a relação do orfismo pitagórico com o platonismo. Por fim, comenta aspectos do texto aristotélico Sobre a memória e a anamnese observando analogias entre a anamnese aristotélica e a descrição do melos na filosofia musical aristoxeniana.
O artigo aborda as relações entre as propostas de encenação de Mário de Andrade e a arte sonora contemporânea, nas edições das peças radiofônicas/podcasts Arte no Rádio #01 e #03.
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