Infectious diseases, especially those affecting the respiratory tract, represent a critical problem to health. Crude methanol and water extracts of 10 Venda plants reported to be used ethnomedically in the treatment of respiratory conditions were assessed for their antimicrobial activity against standard strains and clinical isolates of Candida albicans, Haemophilis influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, and Mycobacterium smegmatis using the disc diffusion assay. Four of the 10 plants tested possessed antimicrobial activity, but no activity against K. pneumoniae was observed. Minimum inhibitory concentrations, as determined by the broth microdilution assay, showed three plants, Securidaca longepedunculata, Syzygium cordatum, and Tabernaemontana elegans, to possess MICs ≤ 1 mg/mL. Phytochemical screening, performed by separation on thin layer chromatography using a variety of mobile phases and visualization with spray reagents as well as UV light showed various classes of compounds in the active extracts. Some of these have been associated with antioxidant activity, as confirmed in this study. Moreover, these extracts showed toxicity in vitro to lymphocytes. Although three plant species with significant antimicrobial activity were identified, there is a need for further scientific evaluation regarding identification of the bioactive constituents, as well as their toxicity.

Ethnopharmacological relevance: Bacterial infections remain a significant threat to human health. Due to the emergence of widespread antibiotic resistance, development of novel antibiotics is required in order to ensure that effective treatment remains available. There are several reports on the ethnomedical use of Tabernaemontana elegans pertaining to antibacterial activity.Aim of the study: The aim of this study was to isolate and identify the fraction responsible for the antimicrobial activity in T. elegans (Stapf.) root extracts. Materials and methods:The active fraction was characterised by thin layer chromatography (TLC) and gas chromatography -mass spectrometry (GC-MS).Antibacterial activity was determined using the broth micro-dilution assay and antimycobacterial activity using the BACTEC radiometric assay. Cytotoxicity of the crude extract and fractions was assessed against primary cell cultures; lymphocytes and fibroblasts; as well as a hepatocarcinoma (HepG2) and macrophage (THP-1) cell line using the Neutral Red uptake and MTT assays. Results:The crude root extracts were found to contain a high concentration of alkaloids (1.2% w/w). GC-MS analysis identified the indole alkaloids, voacangine and dregamine, as major components. Antibacterial activity was limited to the Grampositive bacteria and Mycobacterium species, with MIC values in the range of 64 -256 μg/ml. When combined with antibiotics, additive antibacterial effects were observed. Marked cytotoxicity to all cell lines tested was evident in the MTT and Neutral Red uptake assays, with IC 50 values < 9.81 μg/ml. 3Conclusions: This study confirms the antibacterial activity of T. elegans and supports its potential for being investigated further for the development of a novel antibacterial compound.