Ethnobotanical indices are widely used to quantify cultural importance of plants in social studies. This study aims to show users of ethnobotanical indices the effect of sample variation and what methodological approach can be used to circumvent the problems related to sample variation. The methods used are to write an algorithm and used to simulate different sample sizes from which four ethnobotanic indices selected for the present study were estimated. Results showed the instability of the ethnobotanical indices under variations in the size of informants. It proposes bootstrapping as a statistical aid tool to remove the sample size effect in quantitative ethnobotany. For the indices used in the present study 1000 re-samplings eliminated the effect of sample size on the value of the indices. Researchers will have to take this new approach into account in order to calculate more precise ethnobotanical indices in order to better appreciate the cultural importance of plants.

The glutathione-S-transferase genes mainly the GSTM1 and GSTT1 alleles are responsible for the synthesis of detoxication enzymes that can remove toxic substances. The objective of this study is to seek changes in the genetic polymorphism of glutathione-S-transferase GSTM1 and GSTT1 in motorcycle drivers exposed to BTEX. Our study group consists of 60 motorcycle drivers including 30 professional and 30 non-professional. Blood samples were preleveled from the study population in the EDTA tubes and DNA was extracted by the phenol/chloroform method. The PCR technique was used to determine the presence or absence of genes. Our results showed that the percentage of GSTM1 null genotype has a statistically significant difference (P = 0.02), while the percentage of GSTT1 null genotype was non-significant (P = 0.76) between the two groups. The percentage of deletion of both genes is higher in professional than non-professional motorcycle drivers. Air pollution in Cotonou by BTEX seems to influence the deletion of the GSTM1 and GSTT1 genes at a higher percentage among professional than non-professional motorcycle drivers.