This study aimed to investigate the effects of single local vibration (LV) with and without blood flow restriction (BFR) on muscle activity and hormonal responses. A total of 12 physically inactive males were exposed to 10 sets of intermittent LV (35-40 Hz) on unilateral mid-quadriceps in the supine lying position and LV + BFR (inflated to 140 mmHg) sessions in a repeated-measures randomized crossover design, with a 1-week interval separating the sessions. The results indicated that the electromyography values from the rectus femoris during LV + BFR were greater than those during LV (p < 0.05). LV + BFR caused a minor increase in the lactate (LA) response (p < 0.05); LV with or without BFR failed to elicit change in growth hormone (GH) and testosterone (T) levels (p > 0.05). Cortisol (C) levels were decreased postexercise in both the sessions (p < 0.05). In conclusion, BFR elicited higher increase in muscle activity and metabolic response, but it did not induce hormonal responses. The exposure of LV and LV + BFR may only have a relief effect as detected by the reduction in C levels, probably because the LV did not elicit sufficient stimulus to the muscles.