Anal cancer is relatively rare; however, its incidence has increased in recent years. Several risk factors are associated with the development of anal cancer, including age older than 50 years, low-fiber diet, chronic anal fistulas, smoking, multiple partners, anal intercourse practice, Human Immunodeficiency Virus infection and immunosuppression. However, the presence of human papillomavirus represents the main risk factor for the development of anal cancer. The aim of this study was to evaluate the clinicopathological aspects of a series of patients with anal carcinomas diagnosed in Hospital Araújo Jorge, Goiânia-Goiás, as well as the prevalence of human papillomavirus genome in these tumors. Clinical, pathological and socio-demographic data were collected from the respective medical files and paraffin blocks containing anal carcinomas specimens were used for DNA extraction and detection of human papillomavirus, by means of polymerase chain reaction, using short PCR fragment primers. Forty-three cases were selected and had the data analyzed, while 38 cases were tested for human papillomavirus genome detection. Among the evaluated patients, 62.8% were women; 53.4% of tumors were squamous cell carcinoma and 46.5% of the patients were aged between 60 and 75 years. Risk factors, such as smoking (39.5%) and alcoholism (20.9%) were recorded in the studied group. Lymph node metastases were detected in 30.2% of cases and 7.0% had distant metastasis. The detection of human papillomavirus DNA was positive in 76% of cases assessed and this was significantly associated with squamous cell carcinomas. Aggressive behavior and advanced stage of anal cancer described in this study highlight the need for preventive measures that contemplate these tumors, including vaccination against human papillomavirus.

Resumo. O artigo 158 do Código de Processo Penal Brasileiro (CPP) determina que quando a infração deixar vestígios será indispensável o exame do corpo de delito. Porém, muitas impressões digitais que chegam à seção de confronto da Polícia Técnico-Científica de Goiás (SPTC), não apresentam condições de análises por estarem borradas ou incompletas. Por este motivo, este trabalho buscou: analisá-las por microscopia óptica, verificando a presença de células da epiderme; extrair DNA de impressões latentes aderidas a diferentes suportes e daquelas reveladas com spray de ninidrina especial para documentos ou impregnadas com pó de cor preto volcano "HI-FI". Este estudo detectou células descamativas em 98% das lâminas coradas por Leishman. Em outro experimento, foram extraídas moléculas de DNA de sessenta e nove amostras, depositadas em cinco suportes diferentes (alumínio, madeira, papel, plástico e vidro) obtendo concentrações variadas entre 0,3 ng/µL à 25,4 ng/µL. Isoladamente, a madeira foi o suporte que apresentou maior concentração média de DNA (10,67 ng/µL). Ao extrair DNA de impressões reveladas com ninidrina ou impregnadas pelo pó preto volcano "HI-FI" (leve) da empresa SIRCHIE, obteve se concentração de DNA amplificável em 70% e 60% das amostras, respectivamente. O presente trabalho corrobora com vários estudos que já demonstraram ser possível