SummaryTo evaluate the results of the application of intrastromal voriconazole in the topical treatment of resistant Aspergillus fumigatus. We present an interventional case report. A 50 mg/0.1 mL solution of voriconazole was injected into the corneal stromal tissue around the infiltration and put on topical voriconazole (1%) after the ulcer had failed to respond to 0.15% (1.5 mg/ml) topical amphotericin B hourly and systemic convantional amphotericin B (0.1 mg/kg) per day. Clinical outcomes were assessed at each visit. After the intervention, a faster reduction in the corneal infiltration and a dramatic therapeutic reponse was observed in the patient.