Gallic acid (3,4,5-trihydroxybenzoic acid, GA), a natural phenolic acid has been reported as a strong antioxidant. Therefore the present study was designed to evaluate the effects of GA and dodecyl gallate (DGA) against acute and chronic carbon tetrachloride (CCl 4 )-induced hepatotoxicity. For acute model, rats were orally treated with GA and DGA for 7 d prior to CCl 4 by intraperitoneally (i.p.) injection. For the chronic model, rats were orally treated with GA or DGA and CCl 4 i.p. twice a week for four weeks. In both acute and chronic models, the CCl 4 -treated groups showed significantly increase in serum hepatic enzyme activities and histopathologic alterations, as well as a disruption in antioxidative status. In contrast, the treatment with GA and DGA restored serum hepatic enzymes activities, improved histopathologic alterations, increased glutathione (GSH) and decreased lipid peroxidation levels. The activities of liver antioxidant enzymes were increased by GA and DGA only in acute model. The expression of p53 gene increased about 3.5 times after GA and DGA treatments, which could result in cell death of damaged hepatocytes preventing of a lifelong liver failure. Thus, these results suggest that GA and DGA has the potential to prevent liver damages as the case of fibrosis condition.

O glifosato é um herbicida utilizado em vários tipos de culturas e é considerado de baixa toxicidade, no entanto, os mecanismos de toxicidade das formulações ainda não estão bem esclarecidos. O objetivo do estudo foi avaliar os efeitos do Trop® e do glifosato sobre os parâmetros oxidativos, potencial genotóxico, função renal e  hepática, em animais submetidos ou não ao tratamento com vitamina E. Utilizou-se camundongos Swiss machos albinos, divididos em nove grupos (n=8): Controle (salina); glifosato (50 e 500 mg/kg); Trop® (50 e 500 mg/kg); Glifosato e vitamina E (20 e 200 mg/kg); Trop® e vitamina E (20 e 200 mg/kg). O tratamento foi realizado em dose única via oral. Após 48 horas, os animais foram sacrificados o sangue e o fígado coletados. O potencial genotóxico foi avaliado pela técnica de micronúcleos, a peroxidação lipídica através das espécies reativas ao ácido tiobarbitúrico (TBARS); a capacidade antioxidante pela concentração de glutationa reduzida (GSH) e catalase (CAT); a função renal através das dosagens de uréia e creatinina e a função hepática através das dosagens da Alanina-amino-transferase (ALT) e Aspartato-amino-transferase (AST). Os resultados mostram que não houve um aumento no número de micronúcleos nos eritrócitos demonstrando assim que não houve danos no DNA. Os níveis de TBARS e atividade da CAT teve aumento significativo em comparação aos animais controle e redução da GSH. Os valores de uréia e creatinina tiveram um aumento significativo quando os animais foram tratados com as doses de 500mg/Kg, no entanto, estes efeitos foram revertidos quando os animais foram tratados com combinação de vitamina E 200 mg/Kg. As dosagens de AST e ALT não evidenciaram alterações entre os grupos tratados. Os resultados mostram que uma exposição única ao glifosato pode causar dano oxidativo hepático e alteração na função renal que pode ser revertido pela administração de anti-oxidantes em particular a vitamina E.Palavras-chave: Glifosato. Dano Oxidativo. Potencial Genotóxico. TROLOX OF PROTECTIVE ACTION FRONT OXIDATIVE DAMAGE INDUCED BY GLYPHOSATE AND TROP® IN ANIMAL MODELABSTRACT: Glyphosate is an herbicide used in various types of cultures, considered to have low toxicity, however, the mechanisms of toxicity of the formulations are not wellunderstood. Theaim of the study was to evaluate the effects of Glyphosate on Trop® and oxidative parameters, potential genotoxic an drenal function in animals submitted or not to treatment with vitamin E. Male albino Swiss mice were used divided into nine groups (n=8). The control group (saline); Glyphosate (50 and 500mg/kg); Trop® (50 and 500mg/kg); Glyphosate + Vitamin E (20 and 200 mg/kg); Trop® + Vitamin E (20 and 200 mg/kg). Treatment was performed in a single oral dose, 48 hours after treatment the animals were sacrificed the blood and liver were collected. The genotoxic potential was assessed by micronucleus technique. The evaluation of lipid peroxidation was accomplished by determination of thiobarbituric acid reactive species (TBARS). The antioxidant status was evaluated by concentration of reduced glutathione (GSH) and the catalase (CAT) activity. The renal function was evaluated through the urea and creatinine dosages. The results show that there was not increase in the number of micronuclei in erythrocytes of the peripheral blood of animals treated with Glyphosate or Trop® thus demonstrating that DNA damage has not occurred when animals were treated with the herbicide. However, the oxidative parameters were altered, the levels of TBARS and CAT activity had a significant increase compared to control animals and significantly reduced GSH. The values of urea and creatinine were significantly higher when animals were treated with doses of 500mg/Kg, however, these effects were reversed when the animals were treated with combination of vitamin E 200mg/Kg. The results show that even a single exposure to Glyphosate may cause hepatic oxidative and renal damage which can be reversed by the administration of antioxidants in particular vitamin E.Keywords: Glyphosate. Oxidative damage. Genotoxic potential.