Under these conditions, the inductive capacity of rhBMP-2 for cell differentiation was inhibited. There was a slight acceleration in tissue healing in the group that received irradiation with low-power laser light.

Objective: The aim of this study was to assess the histological reaction of the subcutaneous tissue of rats after the implantation of natural inorganic mineral scaffold from a calf femur containing recombinant human bone morphogenetic protein (rhBMP-2) and irradiated with low-power laser light. Material and methods: Sixteen Wistar rats were incised in the torso in a medial-longitudinal orientation, and the subcutaneous tissue of the left and right sides of the incision was pulled apart for implantation of the inorganic bone scaffold containing rhBMP-2. Diode laser light was applied to the right side implant at a dose of 8 J/cm2 for 3 minutes, forming two groups: G1 (control) and G2 (irradiated with laser). Implants and surrounding tissue were removed from four animals on days 7, 21, 40 and 112 for microscopic study. The histological results were assessed by means of grading (0 = absence, 1 = slight presence, 2 = representative and 3 = very representative), considering the following events: formation of osteoid structure, acute inflammation, chronic inflammation, fibrin deposition, neovascularization, foreign body granuloma and fibrosis. Results: The results showed no statistically significant differences in each of the four periods when the two groups were compared (p>0.05 – Mann-Whitney’s test). Conclusion: The natural inorganic scaffold from a calf femur with rhBMP-2 was a biocompatible combination. Under these conditions, the inductive capacity of rhBMP-2 for cell differentiation was inhibited. A slight hastening of tissue healing was shown in the group that was irradiated with low-power laser light.

The aim of this in vitro study was to evaluate the tensile bond strength of a self-etching adhesive system to three different dentinal substrates. Primary molar teeth that had been recently exfoliated (RE), with unknown time of exfoliation (UT), and extracted due to prolonged retention (PR) were used for this investigation. Ten primary molar teeth of each group were cut in the middle following the mesio-distal direction, creating a total of twenty specimens per group. The specimens were included in acrylic resin and had a flat dentin surface exposed. The self-etching adhesive system was applied to this surface and a 3-millimeter high cone with diameter of 2 mm in the adhesion area was constructed using composite resin. The specimens were stored in distilled water at 37°C for 24 hours. Fifteen specimens of each substrate were used for the tensile bond test (n = 15) and 5 had the interface analyzed by scanning electron microscopy (SEM). The data was examined by one-way ANOVA and presented no significant differences between groups (p = 0.5787). The mean values obtained for RE, UT and PR were 18.39 ± 9.70, 19.41 ± 7.80, and 23.30 ± 9.37 MPa, respectively. Any dentinal substrates of primary teeth studied are safe for tensile bond strength tests with adhesive systems.

Objetivo: Avaliar a capacidade antimicrobiana de três cimentos de ionômero de vidro tendo como grupo controle uma pasta de hidróxido de cálcio, sobre cultura bacteriana mista proveniente da cavidade bucal de crianças. Método: Os materiais empregados nos ensaios laboratoriais foram: Vidrion R (SS White, Brasil), Ketac Molar (Espe 3M), Meron R (Voco) e Biocal (Biodinâmica). Para verificar esta ação antimicrobiana, foi utilizada cultura mista coletada do biofilme de quatro pacientes pediátricos da disciplina de odontopediatria e utilizado o teste de difusão com Agar sangue. Os materiais em forma de corpos de prova foram acomodados em escavações circulares realizadas no meio de cultura, onde foram semeadas as diluições das culturas bacterianas. Os halos de inibição foram medidos em milímetros e os resultados submetidos à Análise de Variância (ANOVA) e teste Tukey (p<0,05). Resultados: O Vidrion R (9,26±0,79) e o Ketac Molar (8.96±0,56) não apresentaram diferença estatística significante entre si (p>0,05), porém em todas as outras combinações dos materiais, houve diferenças estaticamente significantes (p<0,05). O cimento Meron R (12,33±2,06) apresentou maiores halos de inibição e os menores foram com o Biocal (5.59±0,76). Conclusão: Todos os materiais estudados apresentaram ação antimicrobiana positiva, sendo que o maior poder antimicrobiano foi demonstrado pelo cimento de ionômero de vidro Meron R.

Objective: The main goals of this report were to describe the surgical procedure and a three-year clinical/radiographic follow-up of a child with compound or combined odontoma. Discussion: In the majority of cases, diagnoses of odontoma are made during the development and eruption of permanent dentition, at the time when the patient is providing the documentation for having orthodontic and/or orthopedic treatment performed. In this case, the diagnosis and treatment were established earlier, before the patient entered the first stage of mixed dentition. This procedure favored normal development of permanent teeth in the maxillary anterior region preventing aesthetic, occlusion and phonation problems. Conclusion: The surgical removal of an odontoma diagnosed early (still in primary dentition), after careful clinical and radiographic analysis of the location, stage of formation and position of the teeth, is an approach which, in the majority of cases, favors eruption and alignment of permanent teeth.