SUMMARYA relationship has been suggested between eggshell colour and female body condition based on the opposing antioxidant properties of the two main eggshell pigments: the antioxidant biliverdin (blue-green) and the pro-oxidant protoporphyrin (brown). We hypothesized that experimentally food-restricted females with low antioxidant capacity would deposit more protoporphyrin and less biliverdin in their eggshells, resulting in eggshells of reduced brightness but increased colour intensity. Two eggs were collected at the beginning and two at the end of a 2week period from each of 24 female Japanese quails that were either food restricted or receiving ad libitum food (i.e. controls) during that time. Reflectance spectra were recorded and analysed using spectral shape descriptors, chromatic and achromatic contrasts were computed accounting for avian visual sensitivities, and eggshell pigments were quantified. We examined both spot and background pigmentation and found no significant effect of food restriction on eggshell reflectance. However, food-restricted females in lower body condition increased the deposition of protoporphyrin and decreased the amount of biliverdin invested in their eggshells. We hypothesize that in species laying brownspotted eggshells, females modulate eggshell pigment investment in response to their body condition. According to this hypothesis, we predict that females maintain eggshell colour to limit visible changes that could be detected by predators and thereby conceal their eggs, although this work has yet to be conducted. We suggest that further experimental work on egg camouflage under different environmental conditions will elaborate on the process of pigment deposition and the physiological costs to females of laying heavily pigmented eggshells.

No abstract

Preen glands exist in almost every bird species and several non-exclusive functions have been proposed for this gland and the oils that it produces. One function generally admitted is that the oily secretions of the preen gland would provide a waterproofing layer when spread over feathers. Alternatively, several authors have proposed that plumage waterproofness is mostly due to the spatial micro-structure of feathers. The purpose of this study was to examine, by manipulating the access to the preen gland, the effect of the preen oil on the plumage waterproofness and condition. To explore this question, we carried out two independent experiments where we temporarily blocked access to the preen gland secretions with a removable mechanism in one group of captive mallards (Anas platyrhynchos), whilst a second group of birds had access to gland secretions. In a long-term experiment (3 months of treatment) and a short-term experiment (10 days), we measured plumage water retention and condition. After 3 months without access to preen glands, we found a significant decrease of plumage condition and an associated increase in plumage water retention. Moreover, we found a significant correlation between plumage condition and water retention ability. In contrast, after 10 days of treatment, no significant effect was found on plumage condition and water retention. Our study shows that preen oil acts to maintain plumage condition and suggests that feather microstructure is essential to maintain plumage waterproofness.