In the present study, the Carica Papaya Seeds (CPS) were extracted by using 95% ethanol with Soxhlet extraction method. After the plant extract was subjected for the sterility test and results showed no growth of bacteria, the plant extract was examined for the phytochemical screening. The antibacterial test was carried out with agar well diffusion via examination of nutrient and tryptic soy agar. MIC test is used to determine whether the bacteria can be inhibited by plant extract concentrations that are achievable and safe in the blood stream, it was conducted by culturing microorganism in liquid media. The antioxidant activity and total phenolic content were carried out to identify the IC50 value and the total amount of phenolic content of CPS. For the phytochemical screening test, the results showed the presence of alkaloids, anthraquinone, flavonoids, and carbohydrate. The DPPH radical scavenging assay and total phenolic test were shown positive for the antioxidant activity of the CPS. As a basis, Folin-Ciocalteu reagent was used to measure the phenolic content of different concentrations of plant extract. In this test, the Gallic acid was used as a standard and the total phenolic content was calculated by using the formula C = (A/B) x dilution factor. In the present study, the total phenolic content found was 6.420 mg GAE/g and 6.097 mg GAE/g for the concentrations of 50 and 200µg/ml respectively. According to the results obtained, the IC50 was calculated for the BHT as well as CPS extract, the value was 84.04 µg/ml and 182.82 µg/ml, respectively. Besides that, the percentage scavenging value obtained for the ethanolic C. papaya seed was inconsistent and not precise, therefore the results cannot be considered or equivalent to the antioxidant activity exerted by the CPS as the results are not reliable. However, it can be concluded that the CPS are exhibiting antioxidant effect as it shows positive results for the Total Phenolic Content (TPC) as well as DPPH scavenging method.