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<p>Human intestinal mucus essentially consists<br />of a network of Mucin2 glycoproteins<br />embedded in many lower molecular<br />weight proteins. This paper contributes to<br />the proteomic study of human intestinal<br />mucus by comparing two sample collection<br />methods (transanal irrigation and brush<br />cytology during proctosigmoidoscopy) and<br />analysis techniques (electrophoresis and<br />digestion in solution). The entire sample<br />collection and treatment process is explained,<br />including protein extraction, digestion and<br />desalination and peptide characterisation<br />using a nanoAcquity UPLC chromatograph<br />coupled to an HDMS spectrometer equipped<br />with a nanoESI source. Collecting mucus via<br />transanal irrigation provided a larger sample<br />volume and protein concentration from a<br />single patient. The proctosigmoidoscopy<br />sample could be analysed via digestion in<br />solution after depleting albumin. The analysis<br />indicates that a simple mucus lysis method<br />can evaluate the electrophoresis and digestion<br />in solution techniques. Studying human<br />intestinal mucus complexes is important<br />because they perform two essential survival<br />functions for humans as the first biochemical<br />and physical defences for the gastrointestinal<br />tract and a habitat for intestinal microbiota,<br />which are primarily hosted in the colon and<br />exceeds the human genetic information and<br />cell number 100- and 10-fold (1).</p>

RESUMENLa miografía por impedancia eléctrica es una técnica novedosa, rápida, fácil de usar, no invasiva e indolora y que permite obtener información sobre la estructura tisular, que no es posible obtener utilizando otras técnicas de electrodiagnóstico. Si bien, aún se trata de una técnica en desarrollo, los estudios y publicaciones en el tema permiten visualizar su potencial de aplicación en la práctica clínica. El propósito del presente trabajo es la difusión de esta nueva herramienta de evaluación, a partir de la revisión de algunos conceptos básicos de bioimpedancia, que permitan la interpretación de los resultados de los estudios publicados sobre el tema, para luego considerar su uso en el diagnóstico y evaluación de ciertas patologías neuromusculares comunes.Palabras clave: bioimpedancia eléctrica, enfermedades neuromusculares, miografía por impedancia eléctrica. ABSTRACTElectrical impedance myography is a novel, fast, easy to use, non-invasive and painless technique that provides information on tissue structure not measurable using other electro-diagnostic techniques. While it is still under development, studies and publications on the subject allow visualizing their potential application in clinical practice. The purpose of this work is the dissemination of this new assessment tool, through a review of some basic concepts on bioimpedance, which allow the interpretation of results found in published studies on the topic, and, subsequently, a review of its uses in the diagnosis and evaluation of common neuromuscular diseases.