mosaic layer can be visualized by the confocal microscope. In light microscopy, diagnostic difficulties are compensated by refocusing during the assessment and by evaluating additional paraffin sections.BCCs located in areas with sebaceous glands were more complicated to diagnose because sebaceous glands and nodular BCC showed similar structures in black-and-white images. One approach to this problem could be the staining of BCC cells with fluorophores as reported recently. 6,7,10 Technical problems are still time consuming and further investigation in the field of CLSM for micrographic surgery is certainly necessary. A larger field-of-view and matching stitch is required to accelerate the scanning of a complete specimen. In recent studies, some advances in instrumentation have been reported but have not yet been clinically validated. [5][6][7] To our knowledge, our study represents the largest CLSM series combined with micrographic surgery published to date. Our results showed a still unreliable association between histological and CLSM images. Further investigations are needed to apply this technology in routine micrographic surgery. However, large-area and high-quality mosaicing might revolutionize micrographic surgery of BCCs, as CLSM might provide a rapid way to analyse tumour excision margins.
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