The inappropriate use of carbapenems in recent decades has begun to lead to an increase in carbapenem-resistant Gram-negative bacilli. Within the mechanisms of resistance, the synthesis of carbapenemases is the most relevant.The objective was to detect the presence of carbapenemases in Gram-negative bacilli resistant to carbapenems isolated from patients of the Belen de Trujillo hospital during the months of July 2018 to July 2019, the cultures were determined by the Modified Hodge Test, as well as the Synergy Test with Boronic Phenyl Acid for the detection of serinocarbapenemases and the Disc Approximation method for the detection of metallocarbapenemases. It was determined that of the total carbapenem resistant found a little more than half were likely producers of carbapenemases being the Serincarbapenemases the most frequently found and Pseudomonas aeruginosaand Acinetobacter baumaniiare the bacilli most frequently found as carbapenemase producers.