The present work looks at the effectiveness of microbound spray‐dried feed (MB‐SD) elaborated with two phycocolloids, sodium alginate (ALG) and kappa‐carrageenan (KAPACA), and no binder at all (SL). Feeding trials were carried out on larval Pacific white shrimp Litopenaeus vannamei from wild broodstock. Nauplii were stocked in 1.5‐L round‐bottomed flasks at a rate of 100 larvae/tank. Thirty thousand cells/mL (70% Chaetoceros muelleri and 30% Isochrysis galbana) were added to the artificial feed treatments as a single dose of live algae (SDLA) administered on the first day of the experiment. The MB‐SD was given at 6‐16 mg/L daily every 4 h from 0800 to 2000 hours. Water was not exchanged during the 10‐d experimental period. The MB‐SD with KAPACA gave the highest survival (61 ± 4.6%), good mean individual dry weight (104 ± 20.9 mg), and high metamorphic rate (81 ± 14%), followed by MB‐SD with ALG (56.9 ± 4.1% survival; 65 ± 10% metamorphic rate) and MB‐SD with SL (49.2 ± 6.2% survival; 76 ± 12% metamorphic rate). The latter feeds did not show a significant difference; however, they were slightly better than live food (algae plus brine shrimp Artemia sp.; 51.3 ± 7.9% survival; 52 ± 8% metamorphic rate). The MB‐SD with KAPACA provided good larval survival and growth and could be used in experimental larviculture.
Abstract— In this work a larval shrimp diet was spray‐dried using a ratio of 75% whey protein concentrate and 25% mesquite gum as wall material, where the sole protein contribution to the diet was the whey protein concentrate. The microencapsulated diet exhibited the following physical properties: mean volumetric particle size of 49.3 om, characteristic floatability time 225.3 min, and density of 606 g/L. Its outer morphology was characterized by a dimpled spherical shape with no evident surface pores or cracks. Inner microstructure showed a central void, with the feed components finely embedded in the wall matrix. Two bioassays were performed with zoea of white shrimp Litopenaeus vannamei. In the first study, microencapsulated diet, and microencapsulated diet plus a single dose of live algae, were compared with a live algae control diet; in the second study, three co‐feeding regimes consisting of microencapsulated diet + 10,30 and 60C. muellericellaL, respectively, were compared with the live algae control diet. In overall terms, the co‐feeding regime with 30 cells/ OL produced larvae with carapace length (0.74‐mm CL) and development index (4.5 DI) significantly superior to that of the live algae treatment (0.70‐mm CL, 4.1 DI; respectively), and yielded similar individual dry weight (34‐Og DW) and survival rate (58%). These results indicate that microencapsulated diets supplemented with live algae can produce responses similar to those of live feed. These results
Los laboratorios no han logrado producir suficientes nauplios de buena calidad por lo que sigue siendo necesario recolectar hembras maduras en alta mar. Sin embargo, no siempre se recolectan hembras en la condición inseminada. En este estudio, se evaluó la recolecta de hembras y producción de nauplios abordo de un barco camaronero durante un viaje comercial como una alternativa para el larvicultivo de Litopenaeus vannamei en el Golfo de Tehuantepec. Se efectuaron arrastres en la tarde hasta el anochecer. El mejor tiempo de arrastre fue de 20 rnin y produjo una captura media de 37 ± 8 camarones adultos con una sobrevivencia de 72 ± 3 %. Se observaron desoves desde las 11:00 PM hasta las 02:00 AM, con un máximo a las 12:00 PM. Se siguieron " 14 desoves en cinco días, durante los cuales se produjeron 2.2 x 106 nauplios con una tasa media de fertilidad y de eclosión de 83 y 75 %, respectivamente. Este trabajo presenta una técnica que puede proveer de simientes para el larvicultivo a partir de los productos reproductivos de camarones capturados por la flota camaronera comercial.
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