Mosquitoes are efficient, militarily relevant vectors of infectious disease pathogens, including many RNA viruses. The vast majority of all viruses are thought to be undiscovered. Accordingly, recent studies have shown that viruses discovered in insects are very divergent from known pathogens and that many of them lack appropriate reference sequences in the public databases. Given that the majority of viruses are likely still undiscovered, environ mental sampling stands to provide much needed reference samples as well as genetic sequences for comparison. In this study, we sought to determine whether samples of mosquitoes collected from different sites (the Caribbean and locations on the US East Coast) could be differentiated using metagenomic analysis of the RNA viral fraction. We report here distinct virome profiles, even from samples collected short distances apart. In addition to profiling the previously known viruses from these samples, we detected a number of viruses that have been previously undiscovered.
Two new scorpion species of the family Buthidae are described herein: Microtityus vieques sp. n. (a member of the subgenus Parvabsonus Armas, 1974) and Tityus alejandroi sp. n. (belonging to the "quisqueyanus" species-group). Both were collected from pitfall traps in the Puerto Rican satellite island of Vieques, in the Greater Antilles, and the former represents the first official record of the occurrence there of the gens Microtityus Kjellesvig-Waering, 1966. As expected, the new species have their closest relatives in Puerto Rico. Fully illustrated descriptions and detailed comparisons are provided. Additionally, two other Puerto Rican species of Tityus currently not assigned to any group are herein formally transferred to the "quisqueyanus" species group: Tityus angelesae Santiago-Blay, 2009 and Tityus juliorum Santiago-Blay, 2009. After this contribution, the scorpion fauna of Puerto Rico is now composed of 19 species.
This article presents a systematic review of the literature on the use of smart meters. The current approach has shown a focus on data acquisition and processing and, mainly, on automation, which are certainly important factors. However, the non-technical approach has been little explored. This condition leads to reflection on aspects beyond the technical scope, such as social, environmental and economic. This work contributes to broadening the vision of researchers in the field of smart grids, presenting the main aspects of the works that the authors' knowledge until now, emphasizing the lack of investigations beyond the purely technical nature, leading to the conclusion that there is still much to explore in the social field through the implementation of smart grids
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